CHANGING THE IDENTITY OF A TRANSFER-RNA BY INTRODUCING A G-U WOBBLE PAIR NEAR THE 3' ACCEPTOR END
CHANGING THE IDENTITY OF A TRANSFER-RNA BY INTRODUCING A G-U WOBBLE PAIR NEAR THE 3' ACCEPTOR END
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DOI：
10.1126/science.2452483
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发表时间：
1988-05-06
期刊：
影响因子：
56.9
通讯作者：
FOSS, K
中科院分区：
文献类型：
--
作者：
MCCLAIN, WH;FOSS, K
虽然蛋白质的遗传密码在20世纪60年代就已经建立，但转运RNA（tRNA）的氨基酸身份的基础仍然未知。为了研究tRNA的身份，将tRNAPhe（苯丙氨酸tRNA）中三个计算机识别位置的核苷酸替换为tRNAAla（丙氨酸tRNA）的相应核苷酸。当在大肠杆菌中作为琥珀抑制因子进行检查时，所产生的tRNA的身份是tRNAAla。
Although the genetic code for protein was established in the 1960''s, the basis for amino acid identity of transfer RNA (tRNA) has remained unknown. To investigate the identity of a tRNA, the nucleotides at three computer-identified positions in tRNAPhe (phenylalanine tRNA) were replaced with the corresponding nucleotides from tRNAAla (alanine tRNA). The identity of the resulting tRNA, when examined as an amber suppressor in Escherichia coli, was that of tRNAAla.