Localization of a pore-forming protein (perforin) in granulated metrial gland cells.
Localization of a pore-forming protein (perforin) in granulated metrial gland cells.
复制标题
造孔蛋白（穿孔素）在粒状子宫腺细胞中的定位。
DOI：
10.1095/biolreprod37.5.1327
复制
发表时间：
1987
影响因子：
3.6
通讯作者：
Young,JD
中科院分区：
文献类型：
--
作者：
Parr,EL;Parr,MB;Young,JD
一些研究表明，子宫腺（MG）的颗粒状子宫腺（GMG）细胞可能是自然杀伤（NK）样细胞。NK细胞的细胞毒性涉及称为穿孔素的成孔蛋白的分泌，其可以粘附在靶细胞膜上以形成被认为参与靶细胞死亡的跨膜孔。在本研究中，我们使用抗穿孔素的抗血清，以确定是否可以检测到这种蛋白质在GMG cells.Mouse子宫血管灌注固定与几种固定剂在妊娠第14天，和组织切片标记的间接免疫荧光法。特异性穿孔素标记在整个MG的GMG细胞中检测到，在基底蜕膜中，在胎盘中。GMG细胞中穿孔素的存在支持了它们可能是NK样细胞的建议。
Several studies have suggested that the granulated metrial gland (GMG) cells of the metrial gland (MG) may be natural killer (NK)-like cells. The cytotoxicity of NK cells involves secretion of a pore-forming protein termed perforin, which can polymerize on the target cell membrane to form transmembrane pores that are thought to be involved in target cell death. In the present study, we used an antiserum against perforin to determine whether this protein can be detected immunohistochemically in GMG cells.Mouse uteri were fixed by vascular perfusion with several fixatives on Day 14 of pregnancy, and tissue sections were labeled by an indirect immunofluorescence method. Specific perforin labeling was detected in GMG cells throughout the MG, in the decidua basalis, and in the labyrinthine placenta. The presence of perforin in GMG cells supports the suggestion that they may be NK-like cells.