Dual drug delivery of vancomycin and imipenem/cilastatin by coaxial nanofibers for treatment of diabetic foot ulcer infections
Dual drug delivery of vancomycin and imipenem/cilastatin by coaxial nanofibers for treatment of diabetic foot ulcer infections
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DOI：
10.1016/j.msec.2021.111975
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发表时间：
2021-02-25
影响因子：
7.9
通讯作者：
Zomorodian, Kamiar
中科院分区：
文献类型：
--
作者：
Davani, Farideh;Alishahi, Mohsen;Zomorodian, Kamiar
糖尿病足溃疡感染是糖尿病患者住院的主要原因。本研究旨在开发万古霉素和亚胺培南/西司他丁核壳纳米纤维，以促进糖尿病足溃疡的治疗。因此，使用静电纺丝技术制备了由聚环氧乙烷、壳聚糖和万古霉素在壳中和聚乙烯吡咯烷酮、明胶和亚胺培南/西司他丁在芯隔室中组成的新型核-壳纳米纤维。采用扫描电子显微镜、透射电子显微镜、傅里叶变换红外光谱、拉伸试验和药物释放试验对纳米纤维进行了表征。采用纸片扩散法评价不同药物浓度的载药纳米纤维对耐甲氧西林金黄色葡萄球菌（MRSA）、大肠杆菌和铜绿假单胞菌的抗菌活性。此外，通过3-（4，5-二甲基噻唑-2-基）-2，5-二苯基-2H-溴化四唑鎓试验研究纤维的细胞毒性。结果表明，所制备的纳米纤维表面光滑，具有核壳结构，几乎没有细胞毒性。纳米纤维毡对沙门氏菌具有显著的抗菌活性。金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌的抑菌圈分别为2.9和2.5cm;对大肠杆菌和铜绿假单胞菌的抑菌圈分别为1.9和2.8cm。关于这些纳米纤维垫的显著抗菌活性，它们可能不仅用于糖尿病足溃疡感染，而且用于其他慢性伤口作为合适的药物递送装置。
Diabetic foot ulcer infections are the main causes of hospitalization in diabetics. The present study aimed to develop vancomycin and imipenem/cilastatin loaded core-shell nanofibers to facilitate the treatment of diabetic foot ulcers. Therefore, novel core-shell nanofibers composed of polyethylene oxide, chitosan, and vancomycin in shell and polyvinylpyrrolidone, gelatin, and imipenem/cilastatin in core compartments were prepared using the electrospinning technique. The nanofibers were characterized using scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, tensile test, and drug release. The antibacterial activity of drug-loaded nanofibers in different drugs concentrations was evaluated against Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, and Pseudomonas aeruginosa by disk diffusion method. Furthermore, the cytotoxicity of fibers was investigated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay. The obtained results showed that the prepared nanofibers were smooth having a core-shell structure with almost no cytotoxicity. The nanofibrous mats exhibited significant antibacterial activity against S. aureus and MRSA with the inhibition zones of 2.9 and 2.5 cm and gram-negative bacteria species of E. coli and P. aeruginosa with the inhibition zones of 1.9 and 2.8 cm, respectively. With respect to the significant antibacterial activities of these nanofibrous mats, they might be used as suitable drug delivery devices not only for diabetic foot ulcer infections but also for other chronic wounds.