Immunohistochemical detection of cdc2 is useful in predicting survival in patients with mantle cell lymphoma
Immunohistochemical detection of cdc2 is useful in predicting survival in patients with mantle cell lymphoma
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DOI：
10.1038/modpathol.3800409
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发表时间：
2005-09-01
期刊：
影响因子：
7.5
通讯作者：
Lai, R
中科院分区：
文献类型：
--
作者：
Hui, D;Reiman, T;Lai, R
最近的基因芯片研究已经报道了几个基因在套细胞淋巴瘤患者中的预后价值。我们的目的是验证这些基因中的三个：α-微管蛋白、cdc2和CENP-F的预后意义。免疫组织化学方法检测α-微管蛋白、cdc2和CENP-F的蛋白表达。对48例福尔马林固定/石蜡包埋的套细胞淋巴瘤的免疫反应性进行了测定。这些结果与增殖标记物Ki67的表达及生存期密切相关。在这48例套细胞淋巴瘤患者中，男性41例，女性7例。确诊时的中位年龄为64.5岁，总的中位生存期为40个月。在良性淋巴结中，cdc2和α-微管蛋白的表达仅局限于生发中心，套层呈阴性。CENP-F的表达更为均匀。在套细胞淋巴瘤中，Ki67与所有三种标记物均显著相关(P50%)，而cdc2与生存期显著相关(P=2)，与较差的临床结局和较高的临床分期(即4vs
Recent cDNA microarray studies have reported the prognostic value of several genes in mantle cell lymphoma patients. We aimed to validate the prognostic significance of three of these genes: alpha-tubulin, cdc2, and CENP-F. The protein expression of alpha-tubulin, cdc2, and CENP-F was assessed using immunohistochemistry. Their immunoreactivity in 48 formalin-fixed/paraffin-embedded mantle cell lymphoma tumors was determined by estimating the percentage of positive cells. These results were correlated with the expression of proliferation marker Ki67 and survival. Of these 48 mantle cell lymphoma patients, 41 were men and seven were women. The median age at time of diagnosis was 64.5 years, and the overall median survival was 40 months. In benign lymph nodes, the expression of cdc2 and alpha-tubulin was restricted to the germinal centers; mantle zones were negative. Expression of CENP-F was more uniformly distributed. In mantle cell lymphoma, Ki67 significantly correlated with all three markers (P50%) and cdc2 (>25%) significantly correlated with shorter survival (P= 2 correlated with worse clinical outcome, and high clinical stage (ie 4 vs