Inactivation of frog virus 3 and channel catfish virus by esculentin-2P and ranatuerin-2P, two antimicrobial peptides isolated from frog skin
Inactivation of frog virus 3 and channel catfish virus by esculentin-2P and ranatuerin-2P, two antimicrobial peptides isolated from frog skin
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DOI：
10.1006/viro.2001.1080
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发表时间：
2001-09-30
期刊：
影响因子：
3.7
通讯作者：
Rollins-Smith, L
中科院分区：
文献类型：
--
作者：
Chinchar, VG;Wang, J;Rollins-Smith, L
虽然很明显，一些两栖动物的数量最近经历了急剧下降，但这些死亡的原因是复杂的，可能涉及多种变量。一种理论认为，环境因素可能引发导致免疫功能下降和对传染病易感性增加的事件。在这里，我们研究了两栖动物先天免疫的一个方面，并表明从蛙类中分离的两种抗菌肽esculentin-2P （E2P）和ranatuerin-2P （R2P）可以灭活青蛙病毒3（一种感染无尾动物的潜在致病性虹膜病毒）和通道鲶鱼疱疹病毒。与哺乳动物抗菌肽相比，E2P和R2P在低至0℃的温度下几分钟内就能抑制病毒的传染性。此外，这些化合物似乎可以直接灭活病毒，而不是通过抑制受感染细胞的复制来起作用。这是首次将两栖动物抗菌肽与两栖动物病毒病原体的保护联系起来的报道，并表明这些化合物可能在维持两栖动物健康方面发挥作用。(C) 2001学术出版社。
While it is clear that some amphibian populations have recently experienced precipitous declines, the causes of those die-offs are complex and likely involve multiple variables. One theory suggests that environmental factors may trigger events that result in depressed immune function and increased susceptibility to infectious disease. Here we examine one aspect of innate immunity in amphibians and show that esculentin-2P (E2P) and ranatuerin-2P (R2P), two antimicrobial peptides isolated from Rana pipiens, inactivate frog virus 3, a potentially pathogenic iridovirus infecting anurans, and channel catfish herpesvirus. In contrast to mammalian antimicrobial peptides, E2P and R2P act within minutes, at temperatures as low as 0 degreesC, to inhibit viral infectivity. Moreover, these compounds appear to inactivate the virus directly and do not act by inhibiting replication in infected cells. This is the first report linking amphibian antimicrobial peptides with protection from an amphibian viral pathogen and suggests that these compounds may play a role in maintaining amphibian health. (C) 2001 Academic Press.