Quantitative analysis of brain gangliosides by high performance liquid chromatography of their perbenzoyl derivatives.
Quantitative analysis of brain gangliosides by high performance liquid chromatography of their perbenzoyl derivatives.
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DOI：
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发表时间：
1985
影响因子：
6.5
通讯作者：
M. Ullman;R. McCluer;Rogers Memorial;E. Kennedy
中科院分区：
文献类型：
--
作者：
M. Ullman;R. McCluer;Rogers Memorial;E. Kennedy
This report describes a convenient, highly sensitive, and reproducible HPLC procedure for the quantitative analysis of gangliosides from brain tissues. The procedure involves the conversion of gangliosides to their perbenzoyl derivatives, isolation of the derivatives on a C18-reversed-phase cartridge, separation of the derivatives on a column (3-micron silica) maintained at an elevated temperature, and UV detection of the derivatives at 230 nm. The convenience of the procedure, its sensitivity, reproducibility, and application to the analysis of gangliosides from tissue sources make it the method of choice for ganglioside quantification in our laboratories. Three aspects of the procedure contribute to its convenience: reaction conditions that lead to single products, a convenient isolation procedure for the derivatives, and chromatographic conditions that provide resolution of the derivatives.
