IDENTIFYING PLANT GENES SHAPING MICROBIOTA COMPOSITION IN THE BARLEY RHIZOSPHERE
IDENTIFYING PLANT GENES SHAPING MICROBIOTA COMPOSITION IN THE BARLEY RHIZOSPHERE
复制标题
鉴定影响大麦根际微生物群组成的植物基因
DOI：
10.1101/2021.12.20.472907
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Escudero-Martinez C
中科院分区：
文献类型：
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作者：
Escudero-Martinez C
利用土壤微生物进行可持续作物生产的先决条件是确定根际（根与土壤之间的界面）中形成微生物群组成的植物基因。在这里，我们使用宏基因组学信息作为外部定量表型来绘制大麦（全球第四大栽培谷物）野生和驯化基因型根际微生物群的宿主遗传决定因素。我们确定了一个小数目的基因座的根际群落的组成上的重大影响。其中之一，命名为QRMC-3 HS，成为微生物群组成的主要决定因素。我们将在QRMC-3 HS上携带对比等位基因并托管对比微生物的土壤生长的姊妹系进行比较根RNA-seq分析。这使我们能够确定三个主要的候选基因，包括一个核苷酸结合亮氨酸丰富的重复序列（NLR）基因在大麦基因组的结构变异的区域。我们的研究结果为作物改良对植物塑造根际微生物的能力的影响提供了见解。
A prerequisite to exploiting soil microbes for sustainable crop production is the identification of the plant genes shaping microbiota composition in the rhizosphere, the interface between roots and soil. Here, we use metagenomics information as an external quantitative phenotype to map the host genetic determinants of the rhizosphere microbiota in wild and domesticated genotypes of barley, the fourth most cultivated cereal globally. We identify a small number of loci with a major effect on the composition of rhizosphere communities. One of those, designated theQRMC-3HS, emerges as a major determinant of microbiota composition. We subject soil-grown sibling lines harbouring contrasting alleles atQRMC-3HSand hosting contrasting microbiotas to comparative root RNA-seq profiling. This allows us to identify three primary candidate genes, including a Nucleotide-Binding-Leucine-Rich-Repeat (NLR) gene in a region of structural variation of the barley genome. Our results provide insights into the footprint of crop improvement on the plant’s capacity of shaping rhizosphere microbes.