Amh dominant expression in Sertoli cells during the testicular differentiation and development stages in the olive flounder Paralichthys olivaceus
Amh dominant expression in Sertoli cells during the testicular differentiation and development stages in the olive flounder Paralichthys olivaceus
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橄榄牙鲆睾丸分化和发育阶段支持细胞中 Amh 的显性表达
DOI：
10.1016/j.gene.2020.144906
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发表时间：
2020
期刊：
影响因子：
3.5
通讯作者：
You Feng
中科院分区：
文献类型：
--
作者：
Wang Wenxiang;Liang Shaoshuai;Zou Yuxia;Wu Zhihao;Wang Lijuan;Liu Yan;You Feng
摘要橄榄比目鱼是一种重要的海洋鱼类，其生长发育存在性别差异。外源因子影响其性腺分化方向的机制尚待阐明。抗苗勒管激素（amh）基因参与鱼类睾丸的分化和维持。本研究的目的是研究比目鱼肌组织和性腺中的表达。定量表达分析结果表明，该基因在睾丸组织中高表达，尤其在I ~ IV期睾丸组织中表达显著（P< 0.05）。原位杂交和免疫组化结果显示，AMH在睾丸精原细胞周围的支持细胞和外周生精小叶中也有表达。在分化期，三苯氧胺（100 ppm）组睾丸中amh的表达高于17β-雌二醇（E2，5 ppm）组卵巢中amh的表达，在雄性分化过程中，三苯氧胺组amh的表达高于17 β-甲基睾酮（MT，5 ppm）组（P <0.05）。原位杂交结果还表明，amh在幼鱼生殖细胞周围的体细胞中也有表达，与成体相似。此外，在20至80 mm的总长度（TL），他莫昔芬组的比目鱼性腺比MT组保持更多的生殖细胞和体细胞。尤其是在60和80 mm TL时，三苯氧胺组生殖细胞和体细胞数量显著高于MT组（P <0.05）。综上所述，amh可能启动睾丸的分化过程，参与睾丸的早期发育和维持。
The olive flounderParalichthys olivaceus, an important marine fish, shows gender differences in growth. The mechanism on its gonadal differentiation direction affected with exogenous factors still needs to be clarified. The anti-Müllerian hormone (amh) gene is involved in fish testicular differentiation and maintenance. The aim of this study was to investigate the expression of the flounderamhin tissues and the gonads. The quantitative expression analysis results showed that it was highly expressed in the testis, especially in the testis at stages I – IV (P< 0.05). Also,amhwas detected in Sertoli cells surrounding spermatogonia and peripheral seminiferous lobule of the testis within situhybridization (ISH) and immunohistochemistry (IHC). During the differentiation period, theamhexpression in the testis of the tamoxifen treatment group (100 ppm) was higher than that in the ovary of the 17β-estradiol (E2, 5 ppm) group, and the expression levels ofamhduring process of the male differentiation in the tamoxifen group were higher than those in the 17ɑ-methyltestosterone (MT, 5 ppm) group (P <0.05). ISH results also exhibited thatamhwas expressed in the somatic cells that surrounded the germ cells of juvenile flounder similar to adult ones. Furthermore, the flounder gonads in the tamoxifen group maintained more germ cells and somatic cells than those in the MT group from 20 to 80 mm total length (TL). Especially, at 60 and 80 mm TL, the numbers of germ and somatic cells in the tamoxifen group were significantly higher than those in the MT group (P <0.05). In summary,amhmight initiate the process of testicular differentiation, and is involved in the early development and maintenance of testis.