A bone marrow fraction enriched for granulocyte-macrophage progenitors gives rise to osteoclasts in vitro.
A bone marrow fraction enriched for granulocyte-macrophage progenitors gives rise to osteoclasts in vitro.
复制标题
富含粒细胞-巨噬细胞祖细胞的骨髓部分在体外产生破骨细胞。
DOI：
10.1016/8756-3282(88)90014-2
复制
发表时间：
1988
期刊：
影响因子：
4.1
通讯作者：
Relfson,M
中科院分区：
文献类型：
--
作者：
Schneider,GB;Relfson,M
骨硬化啮齿类动物骨髓移植的研究为破骨细胞的谱系提供了证据。最近的研究表明，来自正常动物的含有富集的粒细胞集落形成细胞（G-CFC）或粒细胞巨噬细胞集落形成细胞（GM-CFC）的骨髓分离物可以治疗骨骼硬化症，并导致正常破骨细胞的形成。当移植到骨硬化大鼠体内时。巨噬细胞集落形成细胞分离物在该移植系统中无效。对这些发现的一个批评是，骨石化骨和骨髓腔室的微环境可能是独特的，在他们的能力，以诱导这些干细胞分化成破骨细胞。为了验证这一假设，将G-CFC、GM-CFC和M-CFC与来自正常动物的胎儿跖骨共培养。CFC从正常骨髓中分离，使用FITC标记的单克隆抗体针对大鼠Thy 1.1和荧光激活细胞分选。在软琼脂培养中对分离株进行了评价;粒细胞分离株产生的G-CFC占形成的所有集落的74%，并比未分级分离的细胞增强了30倍。混合分离物产生60%的GM-CFC形成的集落，并增强12倍，而巨噬细胞分离物是77%的M-CFC，增强因子为28。将各种CFC分离物或整个单核骨髓与20天的胎鼠跖骨雏形共培养7天，然后制备用于光学和电子显微镜检查。测定各分离株体外产生的破骨细胞数。G-CFC和GM-CFC分离物产生的破骨细胞显著多于对照培养物中发现的破骨细胞（p<0.01），但M-CFC分离物在产生破骨细胞方面无效。这些结果与骨硬化移植系统中报道的结果相同，并支持破骨细胞特异性细胞谱系在M-CFC阶段之前与巨噬细胞谱系不同的观点。
Studies involving bone marrow transplantation of osteopetrotic rodents have provided evidence for the lineage of the osteoclast. Recent investigations have demonstrated that isolates of bone marrow containing an enriched population of granulocyte-colony-forming cells (G-CFC) or granulocyte macrophage-colony-forming cells (GM-CFC) from normal animals cure the skeletal sclerosis and result in the formation of normal osteoclasts when transplanted intoiaosteopetrotic rats. Macrophage-colony-forming cell isolates were ineffective in this transplant system. A criticism of these findings is that the microenvironment of the osteopetrotic bone and the bone marrow compartment may be unique in their ability to induce the differentiation of these stem cells into osteoclasts. To test this hypothesis, G-CFC, GM-CFC, and M-CFC were co-cultured with fetal metatarsal bones form normal animals. The CFC were isolated from normal bone marrow using FITC-labeled monoclonal antibodies directed against rat Thy 1.1 and fluorescence-activated cell sorting. The isolates were evaluated in soft agar culture; granulocyte isolates generated 74% G-CFC of all colonies formed and were enhanced 30 times over unfractionated cells. Mixed isolates generated 60% GM-CFC of the colonies formed and were 12 times enhanced, while macrophage isolates were 77% M-CFC with an enhancement factor of 28. The various CFC isolates or whole mononuclear bone marrow were co-cultured with 20-day fetal rat metatarsal rudiments for 7 days and then prepared for light and electron microscopy. The number of osteoclasts generatedin vitroby each isolate was determined. The G-CFC and GM-CFC isolates generated significantly more osteoclasts than found in the control cultures (p< .01), but the M-CFC isolates were ineffective in giving rise to osteoclasts. These results are equivalent to those reported in the osteopetrotic transplant system, and support the idea that the osteoclast specific cell lineage diverges from the macrophage lineage prior to the M-CFC stage.