Comparative Studies on the Isolation of Membrane Lipoteichoic Acid from Lactobacillus fermenti
Comparative Studies on the Isolation of Membrane Lipoteichoic Acid from Lactobacillus fermenti
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发酵乳杆菌膜分离脂磷壁酸的比较研究
作者：
A. J. Wicken;J. Gibbens;K. Knox
从发酵乳杆菌NCTC 6991完整菌体中分离出含有不同蛋白质量的脂磷壁酸膜，制备方法选择不同，因为它们能够破坏脂磷壁酸与其他膜组分的疏水相互作用。用热水苯酚制备脂磷胆酸收率最高，且该制剂中蛋白质含量极低。用氯仿-甲醇部分去除细胞脂质，然后在100℃下用水提取，得到一种非常有效的免疫原-脂壁酸-蛋白质复合物；免疫原性被证明与蛋白质含量有关，尽管抗体的特异性是针对磷壁酸成分的。
Preparations of membrane lipoteichoic acid containing different amounts of protein were isolated from intact organisms of Lactobacillus fermenti NCTC 6991 by various procedures chosen for their ability to disrupt the hydrophobic interaction of lipoteichoic acid with other membrane components. The highest yield of lipoteichoic acid was obtained with hot aqueous phenol, and this preparation contained very little protein. Partial removal of cell lipids with chloroform-methanol followed by extraction with water at 100 C gave a lipoteichoic acid-protein complex that was a very effective immunogen; immunogenicity was shown to relate to protein content, though the specificity of the antibodies was directed against the teichoic acid component.