Antennal perception of oilseed rape, Brassica napus (Brassicaceae), volatiles by the cabbage seed weevil Ceutorhynchus assimilis (Coleoptera: Curculionidae)
Antennal perception of oilseed rape, Brassica napus (Brassicaceae), volatiles by the cabbage seed weevil Ceutorhynchus assimilis (Coleoptera: Curculionidae)
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DOI：
10.1007/bf02033667
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发表时间：
1995-11-01
影响因子：
2.3
通讯作者：
Woodcock, CM
中科院分区：
文献类型：
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作者：
Blight, MM;Pickett, JA;Woodcock, CM
采用气相色谱-触角电位法（GC-EAG）和气相色谱-单细胞记录法（SCR）研究了油菜气提液中挥发性物质对大豆斑叶蜂触角的影响。通过这些技术和气相色谱-质谱联用（GC-MS）。共鉴定出25个活性成分，包括类异戊二烯、脂肪酸和氨基酸衍生物。后者中的一些，如异硫氰酸酯和甲状腺肿素，可能还有吲哚和苯乙腈，都是芥子油苷的催化剂。通过使用生理学鉴别剂量的EAG和通过SCR研究证实了所鉴定的化合物的电生理活性。EAG和SCR技术的组合使用的重要性得到了证明，因为特定的嗅觉细胞位于五个化合物，没有引起显着的EAG反应。大多数的嗅觉细胞，从单细胞记录获得显示出非常高的特异性，并在许多记录有特定的细胞类型的一致配对。
The response of the Ceutorhynchus assimilis antenna to volatiles in air entrainment-derived extracts of oilseed rape, Brassica napus, was studied using coupled gas chromatography (GC)-electroantennography (EAG) and coupled GC-single cell recording (SCR). By means of these techniques and coupled gas chromatography-mass spectrometry (GC-MS). 25 active compounds were identified, including isoprenoids and compounds derived from fatty acids and amino acids. Some of the latter, the isothiocyanates and goitrin, and probably indole and benzyl cyanide, are catabolites of glucosinolates. The electrophysiological activity of the identified compounds was confirmed by EAG using a physiologically discriminating dose, and by SCR studies. The importance of the combined use of the EAG and SCR techniques was demonstrated, since specific olfactory cells were located for five compounds that did not elicit significant EAG responses. The majority of the olfactory cells from which single cell recordings were obtained showed very high specificity, and in numerous recordings there were consistent pairings of specific cell types.