Dyadobacter luteus sp. nov., isolated from rose rhizosphere soil
Dyadobacter luteus sp. nov., isolated from rose rhizosphere soil
复制标题
藤黄杆菌 sp.
DOI：
10.1007/s00203-019-01738-5
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发表时间：
2020-01-01
影响因子：
2.8
通讯作者：
Liu, Yunpeng
中科院分区：
文献类型：
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作者：
Chen, Lin;Gao, Xu;Liu, Yunpeng
从玫瑰根际土壤中分离到一株革兰氏阴性好氧杆状细菌RS 19（T）。该菌株是嗜冷的，并且在1-37摄氏度的温度范围内显示出良好的生长。TSB琼脂上的菌落为圆形、光滑、粘液样、边缘清晰的凸形和黄色。基于16 S rRNA基因序列的系统发育分析表明，RS 19（T）与嗜冷双孢菌（Dyadenophilus psychrophilus）CGMCC 1.8951（T）和耐碱双孢菌（Dyadenophilus alkalitolerans）CGMCC 1.8973（T）的亲缘关系最近，分别为97.4%和97.1%。与最接近种型菌株的平均核苷酸同一性值小于84.0%。DNA G + C含量为43.1 mol%，主要呼吸性甲基萘醌为MK-7。主要脂肪酸为特征3（C-16：1 omega 7 c和/或C-16：1 omega 6c）、iso-C-15：0、C-16：1 omega 5c和iso-C-17：0 3-OH。基于基因型、表型和化学分类学数据，菌株RS 19（T）不同于双孢属的近缘种。RS 19（T）代表了双头蜱属中的一个新种，并建议将其命名为黄双头蜱Dyadeluteus sp. nov.。模式菌株为RS 19（T）（= CGMCC 1.13719（T）= ACCC 60381（T）= JCM 32940（T））。
A novel Gram-negative, aerobic, rod-shaped bacterium, RS19(T), was isolated from rose rhizosphere soil. The strain was psychrophilic and showed good growth over a temperature range of 1-37 celcius. Colonies on TSB agar were circular, smooth, mucoid, convex with clear edges and yellow. Phylogenetic analysis based on 16S rRNA gene sequences characterized RS19(T) in the genus Dyadobacter and showed that strain RS19(T) was most closely related to Dyadobacter psychrophilus CGMCC 1.8951(T) (97.4%) and Dyadobacter alkalitolerans CGMCC 1.8973(T) (97.1%). The average nucleotide identity values to the closest related species type strains were less than 84.0%. The DNA G + C content was 43.1 mol%, and the predominant respiratory menaquinone was MK-7. The major fatty acids were summed features 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), iso-C-15:0, C-16:1 omega 5c and iso-C-17:0 3-OH. Based on genotypic, phenotypic and chemotaxonomic data, strain RS19(T) is different from closely related species of the genus Dyadobacter. RS19(T) represents a novel species within the genus Dyadobacter, for which the name Dyadobacter luteus sp. nov. is proposed. The type strain is RS19(T) (= CGMCC 1.13719(T) = ACCC 60381(T) = JCM 32940(T)).