MONTE-CARLO CONVOLUTION METHOD FOR SIMULATION AND ANALYSIS OF FLUORESCENCE DECAY DATA
MONTE-CARLO CONVOLUTION METHOD FOR SIMULATION AND ANALYSIS OF FLUORESCENCE DECAY DATA
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DOI：
10.1063/1.1142280
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发表时间：
1991-01-01
影响因子：
1.6
通讯作者：
BARKLEY, MD
中科院分区：
文献类型：
--
作者：
CHOWDHURY, FN;KOLBER, ZS;BARKLEY, MD
提出了一种模拟时间相关单光子计数数据的蒙特卡罗卷积方法。 随机卷积自动产生真实的实验的泊松统计。 新的模拟技术提供了现实的治疗单光子计数实验的各个方面，包括脉冲堆积。 随机卷积也被纳入使用参考荧光团的数据分析技术。 说明性的例子比较蒙特卡罗和传统的模拟方法，并讨论了概念上的差异。
A Monte Carlo convolution method for simulating time-correlated single photon counting data is presented. The random convolution automatically produces the Poisson statistics of the real experiment. The new simulation technique offers realistic treatment of various aspects of the single photon counting experiment, including pulse pileup. The random convolution is also incorporated in a data analysis technique using a reference fluorophore. Illustrative examples comparing the Monte Carlo and conventional simulation methods are given and the conceptual differences are discussed.