IgA autoantibodies to gliadin nonapeptides, tissue transglutaminase and epidermal transglutaminase are associated, but unrelated to neutrophil elastase expression in lesional skin in human dermatitis herpetiformis
IgA autoantibodies to gliadin nonapeptides, tissue transglutaminase and epidermal transglutaminase are associated, but unrelated to neutrophil elastase expression in lesional skin in human dermatitis herpetiformis
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DOI：
10.5114/pdia.2012.30461
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发表时间：
2012-01-01
影响因子：
1.4
通讯作者：
Bowszyc-Dmochowska, Monika
中科院分区：
文献类型：
--
作者：
Gornowicz-Porowska, Justyna;Seraszek-Jaros, Agnieszka;Bowszyc-Dmochowska, Monika
前言：在疱疹样皮炎中，中性粒细胞通过IgA Fc受体(如CD89)和含有IgA1的免疫复合物被激活，可能导致蛋白水解酶的释放，主要是中性粒细胞弹性蛋白酶(NE)，真皮-表皮交界处的破坏和水泡的形成。目的：分析循环中的表皮自身抗体(ETG)水平与组织(TTG)转谷氨酰胺酶(TTG)、醇溶蛋白非肽(NPG)和皮肤NE表达强度之间的关系。用ELISAs法检测血清中抗ETG、tTG、NPG自身抗体的水平。用去甲肾上腺素(NE)单抗免疫组织化学法和数字形态计量学方法检测NE的表达强度。结果：皮肤NE表达强度与血清中抗ETG、tTG、NPG自身抗体水平之间无统计学意义的相关性。抗ETG、tTG、NPG的IgA自身抗体水平与血清中ETG、tTG、NPG的IgA自身抗体水平呈显著正相关。结论：从NE的表达来看，人的中性粒细胞的活化与血清中抗ETG/tTG/NPG的IgA自身抗体水平无关，但这些自身抗体是协同产生的。
Introduction: In dermatitis herpetiformis (DH), activation of neutrophils via IgA Fc receptors (e.g. CD89) and immuno-complexes containing IgA1 may lead to the release of proteolytic enzymes, mainly neutrophil elastase (NE), destruction of the dermal-epidermal junction and blister formation.Aim: To analyze whether there are associations between levels of circulating IgA autoantibodies to epidermal (eTG) and tissue (tTG) transglutaminases, nonapeptides of gliadin (npG) and the intensity of cutaneous NE expression in patients with DH.Material and methods: Altogether, 31 patients with DH were studied. The levels of serum IgA autoantibodies to eTG, tTG and npG were evaluated with ELISAs. The intensity of NE expression was estimated with immunohistochemistry using NE monoclonal antibody and quantitative digital morphometry. Statistical analysis was done using Spearman's rank correlation coefficient.Results: There were no statistically significant correlations between the intensity of cutaneous NE expression and the levels of serum IgA autoantibodies to eTG, tTG, npG in patients with DH. There were statistically significant correlations between the levels of IgA autoantibodies to eTG, tTG, npG.Conclusions: It seems that in human DH the activation of neutrophils, judged by the NE expression, is unrelated to the levels of serum IgA autoantibodies to eTG/tTG/npG, but all those autoantibodies in DH are produced in a coordinated way.