The SUMOylation of Zinc-Fingers and Homeoboxes 1 (ZHX1) by Ubc9 Regulates Its Stability and Transcriptional Repression Activity
The SUMOylation of Zinc-Fingers and Homeoboxes 1 (ZHX1) by Ubc9 Regulates Its Stability and Transcriptional Repression Activity
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DOI：
10.1002/jcb.24579
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发表时间：
2013-10-01
影响因子：
4
通讯作者：
Guo, Deyin
中科院分区：
文献类型：
--
作者：
Chen, Shuliang;Yu, Xiao;Guo, Deyin
锌指和同源异型盒蛋白1（ZHX1）属于ZF（zinc-fingers）类同源异型域转录因子，其功能在很大程度上仍然未知。ZHX1与核因子Y亚基A（NFYA）的激活结构域相互作用，具有转录抑制活性。在这里，我们报告，SUMO-E2结合酶Ubc9被确定为与ZHX 1的相互作用筛选使用酵母双杂交系统。通过共免疫沉淀和共定位测定证实了这种相互作用。进一步的研究表明，ZHX 1被Ubc9 SUMO化，SUMO 1位于K159、K454和K626位点。此外，我们还证明了ZHX1的SUMO化调控了ZHX1的稳定性、泛素化和转录活性。J.细胞。114：2323 - 2333，2013. (c)2013 Wiley Periodicals，Inc.
Zinc-fingers and homeoboxes protein 1 (ZHX1) belongs to the ZF (zinc-fingers) class of homeodomain transcription factors, and its function remains largely unknown. ZHX1 has been previously found to interact with the activation domain of the nuclear factor Y subunit A (NFYA) and to have a transcriptional repression activity. Here, we report that the SUMO-E2 conjugating enzyme Ubc9 was identified to interact with ZHX1 by an interaction screen using a yeast two-hybrid system. This interaction was confirmed by co-immunoprecipitation and co-localization assays. Further study showed that ZHX1 is SUMOylated by Ubc9 with SUMO1 at the sites K159, K454, and K626. Furthermore, we demonstrated that the SUMOylation of ZHX1 regulated the stability, ubiquitination and transcriptional activity of ZHX1. J. Cell. Biochem. 114: 2323-2333, 2013. (c) 2013 Wiley Periodicals, Inc.