Two genes with similarity to bacterial response regulators are rapidly and specifically induced by cytokinin in Arabidopsis
Two genes with similarity to bacterial response regulators are rapidly and specifically induced by cytokinin in Arabidopsis
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DOI：
10.1105/tpc.10.6.1009
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发表时间：
1998-06-01
期刊：
影响因子：
11.6
通讯作者：
Kieber, JJ
中科院分区：
文献类型：
--
作者：
Brandstatter, I;Kieber, JJ
细胞分裂素是植物生长发育的重要调节因子，但其作用机制尚不清楚。利用差异显示技术，我们从黄化的拟南芥幼苗中鉴定出一个能被细胞分裂素快速诱导的基因IBC6。IBC6 mRNA的稳态水平在10分钟内升高的外源性应用细胞分裂素，这种诱导不需要从头蛋白质合成。IBC6不受其他植物激素或光的诱导。鉴定了具有与IBC6高度相似的序列的第二个拟南芥基因。该IBC7基因也由细胞分裂素诱导，尽管动力学稍慢且程度较小。这两个基因的表达模式是相似的，与较高的表达在叶，轴，花和较低的转录水平在根和角果。序列分析表明，IBC6和IBC7与细菌双组分反应调节因子的受体结构域相似。这种同源性，加上以前发表的工作CKI1组氨酸激酶同源物，表明这些蛋白质可能发挥作用，在早期细胞分裂素信号。
Cytokinins are central regulators of plant growth and development, but little is known about their mode of action. By using differential display, we identified a gene, IBC6 (for induced by cytokinin), from etiolated Arabidopsis seedlings, that is induced rapidly by cytokinin. The steady state level of IBC6 mRNA was elevated within 10 min by the exogenous application of cytokinin, and this induction did not require de novo protein synthesis. IBC6 was not induced by other plant hormones or by light. A second Arabidopsis gene with a sequence highly similar to IBC6 was identified. This IBC7 gene also was induced by cytokinin, although with somewhat slower kinetics and to a lesser extent. The pattern of expression of the two genes was similar, with higher expression in leaves, rachises, and flowers and lower transcript levels in roots and siliques. Sequence analysis revealed that IBC6 and IBC7 are similar to the receiver domain of bacterial two-component response regulators. This homology, coupled with previously published work on the CKI1 histidine kinase homolog, suggests that these proteins may play a role in early cytokinin signaling.