Histological criteria for immunological rejection of mouse skin homografts
Histological criteria for immunological rejection of mouse skin homografts
复制标题
小鼠皮肤同种移植物免疫排斥的组织学标准
作者：
M. Wolman;I. Bleiberg;J. Leibovici
通过光学显微镜研究小鼠皮肤同种移植排斥的事件顺序，并与同种移植的反应进行比较。雄性 C3H 小鼠作为供体，Balb/c 小鼠作为受体。在未感染的移植物中，除了与移植物床接壤的边缘区域之外，没有发生炎症细胞对移植物组织的侵袭。免疫排斥最恒定的特征是移植物-宿主边界处的血管闭塞并伴有移植物缺血。这表明皮肤同种移植排斥主要是一种血管现象，最好通过空小静脉和毛细血管的存在来诊断。在可能感染的移植物中，白细胞浸润很明显。没有发现表皮的破坏和炎症浸润是免疫排斥的标准。在邻近移植物的移植床中，肉芽组织含有嗜碱性淋巴细胞和小淋巴细胞以及巨噬细胞。这些细胞倾向于集中在最靠近移植物的地方，并在外部被淋巴细胞包围。巨噬细胞很可能在消化分解产物方面很活跃。
The sequence of events in homograft rejection of skin in mice was studied by light microscopy in comparison to the reactions to isografts. Male C3H mice were used as donors and Balb/c mice were the recipients. In non-infected grafts no invasion of graft tissue by inflammatory cells occurred except for the marginal areas bordering on the graft bed. The most constant feature of immunological rejection was occlusion of blood vessels at the graft-host border with ischaemia of the graft. This indicated that skin homograft rejection is mainly a vascular phenomenon which can be best diagnosed by the presence of empty venules and capillaries. Leukocytic infiltration was prominent in presumably infected grafts. Destruction and inflammatory infiltration of the epidermis was not found to be a criterion for immunological rejection. In the graft bed adjoining the graft the granulation tissue contained basophilic and small lymphocytes, and macrophages. These cell tended to concentrate nearest to the graft and were surrounded on the outside by the lymphoid cells. It is probable that the macrophages were active in digestion of breakdown products.