Typing and surface charges of the variable loop regions of PorB from Neisseria meningitidis.
Typing and surface charges of the variable loop regions of PorB from Neisseria meningitidis.
复制标题
脑膜炎奈瑟菌 PorB 可变环区的分型和表面电荷。
DOI：
10.1002/iub.1508
复制
发表时间：
2016
期刊：
影响因子：
4.6
通讯作者：
and Massari P.
中科院分区：
文献类型：
--
作者：
Stefanelli P.;Neri A.;Tanabe M.;Fazio C.;and Massari P.
PorB是一种具有三聚体β桶结构的pan - Neisserial主要外膜蛋白。每个单体呈现8个质周弯和8个表面暴露的环区，具有序列可变性。PorB通过TLR2依赖性机制诱导宿主细胞反应的激活，可能是由TLR2和PorB表面暴露环之间的静电相互作用介导的。已知环氨基酸序列的可变性会影响细胞对体外PorBin的反应，特别是L5和L7中的残基。在这项工作中，研究了35株入侵性脑膜炎球菌分离株的PorB基因序列和PorB的静电表面电荷，这些分离株属于意大利鉴定的主要克隆复合物和网站http://pubmlst.org/neisseria/上的5个载体基因组。对侵袭性脑膜炎球菌的卟啉编码区进行分析，发现了四个新的等位基因，以及卟啉、血清群和克隆复合物之间的潜在关联。通过计算机模拟和分析这些菌株的PorB静电表面电荷，观察了入侵血清群B和C的PorB之间的环电荷分离。具体来说，血清组B菌株的环1、环4和环7带负电荷，L2和L8大部分为中性，而侵袭性血清组C菌株的PorB表面呈均匀的正电荷。在载体基因组中观察到较高的PorB序列变异性，并且普遍存在负环表面电荷。血清B组和C组侵袭株和携带株的PorB表面电荷差异可能部分影响奈瑟菌与宿主细胞相互作用的结果。©2016 IUBMB生活，68(6):488-495,2016
PorB is a pan‐Neisserial major outer membrane protein with a trimeric β‐barrel structure. Each monomer presents eight periplasmic turns and eight surface exposed loop regions with sequence variability. PorB induces activation of host cell responses via a TLR2‐dependent mechanism likely mediated by electrostatic interactions between TLR2 and PorB surface exposed loops. Variability in the loop amino acid sequence is known to influence cell responses to PorBin vitro, particularly for the residues in L5 and L7. In this work, the sequence of theporBgene and the electrostatic surface charges of PorB from 35 invasive meningococcal isolates belonging to the main clonal complexes identified in Italy and from five carriage genomes available on the website http://pubmlst.org/neisseria/ were examined. Analysis of theporBencoding regions from the invasive meningococci has identified four new alleles and a potential association betweenporBalleles, serogroup, and clonal complexes. Through computer‐based modeling and analysis of the electrostatic surface charges of PorB from these strains, loop charge segregation between PorB from invasive serogroups B and C was observed. Specifically, loops 1, 4, and 7 were negatively charged and L2 and L8 were mostly neutral in serogroup B isolates, while an overall homogeneous positive surface charge was present in PorB from invasive serogroup C strains. A higher PorB sequence variability was observed among carriage genomes, and a general prevalence of negative loop surface charges. The surface charge differences in PorB from serogroups B and C invasive and carriage strains may, in part, influence the outcomes ofNeisseriaeinteractions with host cells. © 2016 IUBMB Life, 68(6):488–495, 2016