OpenSFDI: an open-source guide for constructing a spatial frequency domain imaging system
OpenSFDI: an open-source guide for constructing a spatial frequency domain imaging system
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DOI：
10.1117/1.jbo.25.1.016002
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发表时间：
2020-01-01
影响因子：
3.5
通讯作者：
Roblyer, Darren
中科院分区：
文献类型：
--
作者：
Applegate, Matthew B.;Karrobi, Kavon;Roblyer, Darren
意义：空间频域成像(SfDi)是一种漫反射光学测量技术，可以在逐个像素的基础上量化组织的光吸收(Mu(A))和减少的散射(Mu(S)‘)。在不同波长测量MU(A)，能够在大范围内提取组织发色团的摩尔浓度，提供了一种非接触和无标记的方法来评估组织的存活、氧合、微结构和分子含量。我们在这里介绍了Open SFDI：一种用于构建低成本、小占地、三波长SFDI系统的开源指南，该系统能够定量测定生物组织中的单位(A)和单位(S)以及氧合血红蛋白和脱氧血红蛋白浓度。目的：我们描述了OpenSFDI的设计，并报告了根据OpenSFDI网站上的说明制造的三种不同系统的光学性质提取的准确性和精密度。方法：通过使用OpenSFDI系统和商用SFDI设备测量9个生理相关范围为Mu(A)和Mu(S)‘的组织模拟光学体模来评估准确性。结果：与商业SFDI系统相比，OPEN SFDI系统的MU(A)误差为0+/-6%，MU(S)‘的误差为-2+/-3%。Bland-Altman分析表明，两个系统之间的一致性限度为：亩(A)为+/-0.004 mm(-1)，亩(S)为-0.06~0.10 mm(-1)。Open SFDI系统具有较低的漂移，平均标准偏差分别为0.0007 mm(-1)和0.05 mm(-1)。结论：Open SFDI为寻求利用SFDI进行定量漫反射光学成像的研究小组提供了一个可定制的硬件平台。(C)作者。由SPIE在知识共享署名4.0未移植许可下发布。
Significance: Spatial frequency domain imaging (SFDI) is a diffuse optical measurement technique that can quantify tissue optical absorption (mu(a)) and reduced scattering (mu(s)') on a pixel-by-pixel basis. Measurements of mu(a) at different wavelengths enable the extraction of molar concentrations of tissue chromophores over a wide field, providing a noncontact and label-free means to assess tissue viability, oxygenation, microarchitecture, and molecular content. We present here openSFDI: an open-source guide for building a low-cost, small-footprint, three-wavelength SFDI system capable of quantifying mu(a) and mu(s)' as well as oxyhemoglobin and deoxyhemoglobin concentrations in biological tissue. The companion website provides a complete parts list along with detailed instructions for assembling the openSFDI system.Aim: We describe the design of openSFDI and report on the accuracy and precision of optical property extractions for three different systems fabricated according to the instructions on the openSFDI website.Approach: Accuracy was assessed by measuring nine tissue-simulating optical phantoms with a physiologically relevant range of mu(a) and mu(s)' with the openSFDI systems and a commercial SFDI device. Precision was assessed by repeatedly measuring the same phantom over 1 h.Results: The openSFDI systems had an error of 0 +/- 6 % in mu(a) and -2 +/- 3 % in mu(s)', compared to a commercial SFDI system. Bland-Altman analysis revealed the limits of agreement between the two systems to be +/- 0.004 mm(-1) for mu(a) and -0.06 to 0.1 mm(-1) for mu(s)'. The openSFDI system had low drift with an average standard deviation of 0.0007 mm(-1) and 0.05 mm(-1) in mu(a) and mu(s)', respectively.Conclusion: The openSFDI provides a customizable hardware platform for research groups seeking to utilize SFDI for quantitative diffuse optical imaging. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.