Magnetic immunoassays utilizing magnetic markers and a high-TC SQUID
Magnetic immunoassays utilizing magnetic markers and a high-TC SQUID
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DOI：
10.1109/tasc.2005.849991
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发表时间：
2005-06-01
影响因子：
1.8
通讯作者：
Hamasaki, N
中科院分区：
文献类型：
--
作者：
Enpuku, K;Inoue, K;Hamasaki, N
利用磁性标记物和高T-C SQUID的磁性免疫测定已经进行。我们首先展示了用于灵敏检测来自标记的磁信号的SQUID的设计，其中考虑了信号场的空间分布。利用这种设计，我们可以得到标记物的磁化强度与SQUID检测到的信号通量之间的关系。该关系对于免疫测定的定量评价是重要的。接下来，我们使用剩磁测量方法检测称为IgE的生物材料，其中使用由直径为25 nm的Fe 3 O 4颗粒制成的标记。我们表明，可以检测到2阿摩尔的IgE，这表明本方法的高灵敏度。
Magnetic immunoassays utilizing magnetic markers and a high-T-c SQUID have been performed. We first showed a design of the SQUID for the sensitive detection of the magnetic signal from the marker, where the spatial distribution of the signal field was taken into account. Using the design, we can obtain the relationship between the magnetization of the maker and the signal flux detected with the SQUID. This relationship is important for quantitative evaluation of the immunoassay. Next, we detected biological materials called IgE with a remanence measurement method, where a marker made of Fe3O4 particles with diameter of 25 nm was used. We showed that 2 atto-mol of IgE can be detected, which shows high sensitivity of the present method.