Development of microsatellite markers in the tetraploid fern Ceratopteris thalictroides (Parkeriaceae) using RAD tag sequencing.
Development of microsatellite markers in the tetraploid fern Ceratopteris thalictroides (Parkeriaceae) using RAD tag sequencing.
复制标题
DOI：
10.4238/gmr.15017550
复制
发表时间：
2016-02
期刊：
影响因子：
--
通讯作者：
Yang Xy;Long Zc;A. W. Gichira;Guo Yh;Qing‐Feng Wang;Jin‐Ming Chen
中科院分区：
文献类型：
--
作者：
Yang Xy;Long Zc;A. W. Gichira;Guo Yh;Qing‐Feng Wang;Jin‐Ming Chen
为了了解四倍体蕨类植物角蕨（Ceratopteris thalictroides, Parkeriaceae）的遗传变异性，我们利用限制性内切位点相关DNA （RAD）标记测序技术获得了30个多态性微卫星标记。每个标记共有26个个体进行基因分型。每个位点等位基因数为4 ~ 10个，期望杂合度和Shannon-Wiener指数分别为0.264 ~ 0.852和0.676 ~ 2.032。由于这30个微卫星标记具有高度的遗传变异，它们将为研究沙棘的适应性遗传变异和可持续保护提供有用的工具。
To understand the genetic variability of the tetraploid fern Ceratopteris thalictroides (Parkeriaceae), we described 30 polymorphic microsatellite markers obtained using the restriction site-associated DNA (RAD) tag sequencing technique. A total of 26 individuals were genotyped for each marker. The number of alleles per locus ranged from 4 to 10, and the expected heterozygosity and the Shannon-Wiener index ranged from 0.264 to 0.852 and 0.676 to 2.032, respectively. Because these 30 microsatellite markers exhibit high degrees of genetic variation, they will be useful tools for studying the adaptive genetic variation and sustainable conservation of C. thalictroides.