The role of isoflavone metabolism in plant protection depends on the rhizobacterial MAMP that triggers systemic resistance against Xanthomonas axonopodis pv. glycines in Glycine max (L.) Merr. cv. Osumi
The role of isoflavone metabolism in plant protection depends on the rhizobacterial MAMP that triggers systemic resistance against Xanthomonas axonopodis pv. glycines in Glycine max (L.) Merr. cv. Osumi
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DOI：
10.1016/j.plaphy.2014.05.001
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发表时间：
2014-09-01
影响因子：
6.5
通讯作者：
Ramos-Solano, Beatriz
中科院分区：
文献类型：
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作者：
Algar, Elena;Javier Gutierrez-Manero, F.;Ramos-Solano, Beatriz
大豆（Glycine max L.）Merr.由于其作为植物蛋白的输入以及其对健康的益处，因此在食品和制药工业领域都起着至关重要的作用。此外，IF在植物生长和植物防御中起关键作用，因此，IF的增加对于更好的田间表现是期望的。IF是次生代谢产物，因此是可诱导的，因此寻找有效的药物来增加IF含量是令人感兴趣的。在这些试剂中，植物促生长根际细菌（PGPR）已被用于通过其微生物相关分子模式（MAMP）触发植物次生代谢的系统诱导，所述MAMP适合于植物的受体以启动系统反应。本研究的目的是评估4种PGPR对IF代谢具有对比作用的能力，保护植物免受生物胁迫，并建立IF谱与细菌引发的系统反应之间的关系。显然，这种反应涉及对乙烯的较低敏感性，尽管有效光合作用减少，但生长仅在保护最有效的M84的情况下受到损害。所有菌株均能保护大豆免受轴足黄单胞菌致病变种的侵害。甘氨酸（M84 > N5.18 > Aur9>N21.4），只有M84和N5.18参与IF。N5.18在病原体攻击前刺激IF的积累。M84仅在病原体攻击后引起IF的显著增加，而N21.4引起IF含量的显著增加，而与病原体攻击无关。Aur9不影响IF。这些结果指出，所有4个菌株都具有触发大豆防御代谢的MAMP。由N21.4和Aur9诱导的保护涉及与IF不同的其他代谢物，并且IF在防御中的作用取决于植物的先前代谢状态和细菌MAMP。(C)2014年Elsevier Masson SAS。All rights reserved.
Glycine max (L.) Merr. plays a crucial role in both the field of food and the pharmaceutical industry due to their input as plant protein and to the benefits of isoflavones (IF) for health. In addition, IF play a key role in nodulation and plant defense and therefore, an increase in IF would be desirable for better field performance. IF are secondary metabolites and therefore, inducible, so finding effective agents to increase IF contents is interesting. Among these agents, plant growth promoting rhizobacteria (PGPR) have been used to trigger systemic induction of plant's secondary metabolism through their microbe associated molecular patterns (MAMPs) that fit in the plant's receptors to start a systemic response. The aim of this study was to evaluate the ability of 4 PGPR that had a contrasted effect on IF metabolism, to protect plants against biotic stress and to establish the relation between IF profile and the systemic response triggered by the bacteria. Apparently, the response involves a lower sensitivity to ethylene and despite the decrease in effective photosynthesis, growth is only compromised in the case of M84, the most effective in protection. All strains protected soybean against Xanthomonas axonopodis pv. glycines (M84 > N5.18 > Aur9>N21.4) and only M84 and N5.18 involved IF. N5.18 stimulated accumulation of IF before pathogen challenge. M84 caused a significant increase on IF only after pathogen challenge and N21.4 caused a significant increase on IF content irrespective of pathogen challenge. Aur9 did not affect IF. These results point out that all 4 strains have MAMPs that trigger defensive metabolism in soybean. Protection induced by N21.4 and Aur9 involves other metabolites different to IF and the role of IF in defence depends on the previous metabolic status of the plant and on the bacterial MAMP. (C) 2014 Elsevier Masson SAS. All rights reserved.