DIFFERENTIAL G-PROTEIN-MEDIATED COUPLING OF NEUROTRANSMITTER RECEPTORS TO CA2+ CHANNELS IN RAT DORSAL-ROOT GANGLION NEURONS INVITRO
DIFFERENTIAL G-PROTEIN-MEDIATED COUPLING OF NEUROTRANSMITTER RECEPTORS TO CA2+ CHANNELS IN RAT DORSAL-ROOT GANGLION NEURONS INVITRO
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DOI：
10.1016/0896-6273(89)90185-2
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发表时间：
1989-02-01
期刊：
影响因子：
16.2
通讯作者：
MILLER, RJ
中科院分区：
文献类型：
--
作者：
EWALD, DA;PANG, IH;MILLER, RJ
神经肽Y（NPY）和缓激肽（BK）均能抑制离体大鼠背根神经节（DRG）神经元Ca ~（2+）电流。这两种肽的作用被百日咳毒素处理细胞完全阻断。根据抗原决定簇，DRG细胞至少含有两种百日咳毒素底物，α 1（M_（1，39）kd）和α 2（M_（1，40）kd）。我们检测了三种纯化的牛α亚基（用抗体鉴定为α 1、α 2、α 1和α 2）重建NPY和BK的抑制作用的能力。然而，在BK的情况下，所有三种C蛋白几乎同样有效。然而，用ao可以完全重建NPY的作用，没有一个a亚基产生BK的完全重建。然而，a和uiz的组合能够完全重建BK的作用。因此，几种C蛋白可以影响这些细胞中Ca*+通道的调节。然而，神经递质可能在用于实现这种调节的G蛋白或G蛋白组合中具有选择性。
The peptides neuropeptide Y (NPY) and bradykinin (BK) both inhibited Ca*+ currents in rat dorsal root ganglion neurons (DRG) in vitro. The effects of both peptides were completely blocked by treatment of cells with pertussis toxin. Based on antigenic determinants, DRG cells contained at least two pertussis toxin substrates, a,(M,, 39kd) and oiz (M,, 40 kd). We examined the ability of three purified bovine a subunits (identified with antibodies as a,, oil, and ui2) to reconstitute the inhibitory effects of NPY and BK. Reconstitution of NPY effects occurred according to the potency series o,> uir>> aiz. However, in the case of BK all three C proteins were approximately equally effective. Whereas complete reconstitution of NPY effects could be obtained with ao, no single a subunit produced complete reconstitution of BK. Combinations of a, and uiz, however, were able to completely reconstitute the effects of BK. Thus several C proteins can effect the regulation of Ca*+ channels in these cells. However, neurotransmitters may be selective in the G proteins or combinations of G proteins utilized to achieve this regulation.