Multifocal structure of the T cell - dendritic cell synapse
Multifocal structure of the T cell - dendritic cell synapse
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DOI：
10.1002/eji.200425857
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发表时间：
2005-06-01
影响因子：
5.4
通讯作者：
Trautmann, A
中科院分区：
文献类型：
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作者：
Brossard, C;Feuillet, V;Trautmann, A
对小鼠幼稚T细胞和成熟树突状细胞之间形成的免疫突触的结构进行了定量分析。无抗原形成的突触的免疫荧光图像显示CD3和LFA-1的弥漫性突触积累。在电子显微镜下，这些无抗原的突触呈现出许多紧密的结合（裂缝大小类似于15纳米），沿着突触。这些紧密的结合覆盖了抗原依赖性突触的明显更大的表面部分。在免疫荧光中，抗原依赖性突触显示CD3和LFA-1的多个斑块具有可变的重叠。PKC θ和talin也有类似的分布。即使树突状细胞被细胞骨架毒物麻痹，也很少观察到典型突触的同心组织特征。在T-DC突触中，相互作用表面由几十个亚微米级的接触点组成，CD3和LFA-1没有大规模的分离。作为比较，在T-B突触中，免疫荧光经常观察到CD3的中心簇，电子显微镜显示一个中心紧密的相对位置。我们的数据表明，将同心圆结构视为“成熟突触”而将多焦结构视为不成熟是不恰当的。
The structure of immunological synapses formed between murine naive T cells and mature dendritic cells has been subjected to a quantitative analysis. Immunofluorescence images of synapses formed in the absence of antigen show a diffuse synaptic accumulation of CD3 and LFA-1. In electron microscopy, these antigen-free synapses present a number of tight appositions (cleft size similar to 15 nm), all along the synapse. These tight appositions cover a significantly larger surface fraction of antigen-dependent synapses. In immunofluorescence, antigen-dependent synapses show multiple patches of CD3 and LFA-1 with a variable overlap. A similar distribution is observed for PKC theta and talin. A concentric organization characteristic of prototypical synapses is rarely observed, even when dendritic cells are paralyzed by cytoskeletal poisons. In T-DC synapses, the interaction surface is composed of several tens of submicronic contact spots, with no large-scale segregation of CD3 and LFA-1. As a comparison, in T-B synapses, a central cluster of CD3 is frequently observed by immunofluorescence, and electron microscopy reveals a central tight apposition. Our data show that it is inappropriate to consider the concentric structure as a "mature synapse" and multifocal structures as immature.