ANALYSIS OF MOLECULAR MASSES AND OLIGOMERIC STATES OF PROTEIN COMPLEXES BY BLUE NATIVE ELECTROPHORESIS AND ISOLATION OF MEMBRANE-PROTEIN COMPLEXES BY 2-DIMENSIONAL NATIVE ELECTROPHORESIS
ANALYSIS OF MOLECULAR MASSES AND OLIGOMERIC STATES OF PROTEIN COMPLEXES BY BLUE NATIVE ELECTROPHORESIS AND ISOLATION OF MEMBRANE-PROTEIN COMPLEXES BY 2-DIMENSIONAL NATIVE ELECTROPHORESIS
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DOI：
10.1006/abio.1994.1112
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发表时间：
1994-03-01
影响因子：
2.9
通讯作者：
VONJAGOW, G
中科院分区：
文献类型：
--
作者：
SCHAGGER, H;CRAMER, WA;VONJAGOW, G
蓝色原生电泳是一种“电荷转移”方法，用于从生物膜中分离原生膜蛋白复合物，也可在固定pH为7.5的条件下分离酸性和碱性水溶性蛋白质。与十二烷基硫酸钠二维电泳相结合，它提供了一种分析方法，用于测定非解离复合物的分子质量和低聚状态，亚基组成。以及纯度和亚复合物的检测。该方法应用于细胞色素bc/bf络合物的分析。将一种新型的无色天然聚丙烯酰胺凝胶电泳（CN-PAGE）与蓝色天然BN-PAGE相结合，开发了一种适用于制备高纯度膜蛋白复合物的二维天然技术。(C) 1994学术出版社，Inc.
Blue native Electrophoresis is a ''charge shift'' method developed for isolation of native membrane protein complexes from biological membranes that also separates both acidic and basic water-soluble proteins at a fixed pH of 7.5, In combination with a second dimension sodium dodecylsulfate electrophoresis it provides an analytical method for the determination of molecular mass and oligomeric state of nondissociated complexes, of subunit composition, and of degree of purity and for the detection of subcomplexes. The method was applied to analysis of cytochrome bc/bf complexes. By combination of a novel colorless native polyacrylamide gel electrophoresis (CN-PAGE) with blue native BN-PAGE, a two-dimensional native technique was developed that is suitable for preparation of highly pure membrane protein complexes. (C) 1994 Academic Press, Inc.