Sample preparation for many-reactant bioassay on DMFBs using common dilution operation sharing
Sample preparation for many-reactant bioassay on DMFBs using common dilution operation sharing
复制标题
使用共同稀释操作共享对 DMFB 进行多反应物生物测定的样品制备
DOI：
10.1109/iccad.2013.6691180
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Juinn
中科院分区：
文献类型：
--
作者：
Chia;Hao;Tung;Juinn
样品制备是大多数生化应用中必不可少的处理步骤。将各种反应物混合在一起，以产生目标浓度的溶液。由于反应物通常在生物测定中取得明显的成本，因此应尽可能最大程度地减少其使用情况。在本文中，我们建议使用数字微流体生物芯片（DMFBS）上的常见稀释操作共享，以许多反应物（DMFBS）准备许多反应物。 CODOS首先表示给定的目标浓度作为配方矩阵，然后识别矩阵中的矩形，每个矩形都表示反应物最小化的稀释操作共享的机会。实验结果表明，与单目标样品制备中的位扫描方法相比，CODO可以实现多达27％的反应物节省。此外，即使没有开发CODOS用于多目标样品制备，它仍然胜过最近最新的算法RSMA。因此，令人信服的是，CODOS是对多重样品制备的更好替代方法。
Sample preparation is an essential processing step in most biochemical applications. Various reactants are mixed together to produce a solution with the target concentration. Since reactants generally take a notable part of the cost in a bioassay, their usage should be minimized whenever possible. In this paper, we propose an algorithm, CoDOS, to prepare the target solution with many reactants using common dilution operation sharing on digital microfluidic biochips (DMFBs). CoDOS first represents the given target concentration as a recipe matrix, and then identifies rectangles in the matrix, where each rectangle indicates an opportunity of dilution operation sharing for reactant minimization. Experimental results demonstrate that CoDOS can achieve up to 27% of reactant saving as compared with the bit-scanning method in single-target sample preparation. Moreover, even if CoDOS is not developed for multi-target sample preparation, it still outperforms the recent state-of-the-art algorithm, RSMA. Hence, it is convincing that CoDOS is a better alternative for many-reactant sample preparation.
影响因子：
7.4
作者：
Chatterjee, Debalina;Ytterberg, A. Jimmy;Garrell, Robin L.
通讯作者：
Garrell, Robin L.
影响因子：
4.7
作者：
Welch, Erin R. Ferguson;Lin, Yan-You;Madison, Andrew;Fair, Richard B.
通讯作者：
Fair, Richard B.