A new cholesterol derivative suitable for transfecting certain type of cells in the presence of 10% serum
A new cholesterol derivative suitable for transfecting certain type of cells in the presence of 10% serum
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DOI：
10.1038/sj.cgt.7700167
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发表时间：
2000-04-01
影响因子：
6.4
通讯作者：
Szala, S
中科院分区：
文献类型：
--
作者：
Sochanik, A;Kaida, I;Szala, S
我们开发了一种新的阳离子脂质适合用作DNA载体在10%的血清的存在下。新化合物（简称Arg-Chol）含有胆固醇和由甘氨酸和空间保护的精氨酸组成的二肽。将基于这种新试剂的脂质体与使用其他胆固醇阳离子衍生物制成的脂质体的报告基因转染效率进行比较。脂质复合物配制与新合成的脂质介导的B16（F10）小鼠黑色素瘤细胞在10%血清的存在下，比其他细胞系更有效地体外转染，并与其他胆固醇衍生物相比研究。
We developed a new cationic lipid suitable for use as a DNA carrier in the presence of 10% sera. The novel compound (abbreviated as Arg-Chol) contains cholesterol and a dipeptide consisting of glycine and sterically protected arginine. The efficiency of reporter gene transfection using liposomes based on this new reagent was compared with that of liposomes made with other cationic derivatives of cholesterol. Lipoplexes formulated with the newly synthesized lipid mediate in vitro transfection of B16(F10) murine melanoma cells in the presence of 10% sera more efficiently than in other cell lines and compared with other cholesterol derivatives studied.