CD44 clustering is involved in monocyte differentiation
CD44 clustering is involved in monocyte differentiation
复制标题
CD44 簇参与单核细胞分化。
DOI：
10.1093/abbs/gmu042
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发表时间：
2014-07-01
影响因子：
3.7
通讯作者：
Gao, Feng
中科院分区：
文献类型：
--
作者：
Zhang, Guoliang;Zhang, Huizhen;Gao, Feng
在生理和病理条件下，单核细胞向巨噬细胞分化是一个重要的过程，但单核细胞分化的潜在机制尚不完全清楚。一些黏附分子已被报道在细胞分化中发挥重要作用。CD44是一种重要的黏附分子，介导细胞与细胞、细胞与基质的相互作用，参与多种细胞功能。由于CD44在单核细胞和巨噬细胞中表现出不同的激活状态，我们认为CD44可能参与了单核细胞的分化。在本研究中，我们探讨了CD44在单核细胞分化中的作用，并进一步研究了其参与的机制。用佛波酯(PMA)诱导THP-1细胞(人单核细胞白血病细胞系)，建立体外单核细胞分化模型。结果发现，在分化过程中CD44的表达和与透明质酸的结合量显著增加，CD44的分布转变为簇状分布。阻断CD44可抑制PMA诱导的CD44聚集和CD44高表达，从而抑制CD14的表达。PMA诱导的ERK1/2信号的磷酸化也通过阻断CD44而被抑制。提示CD44参与了单核细胞的分化。CD44激活后单核细胞分化的机制可能包括CD44的高表达和聚集，进而导致ERK1/2的磷酸化。
Differentiation of monocytes into macrophages is an important process under physiological and pathological conditions, but the underlying mechanism of monocyte differentiation is not completely clear. Some adhesion molecules have been reported to play an important role in cell differentiation. CD44 is an important adhesion molecule that mediates cell-cell and cell-matrix interaction, and participates in a wide variety of cellular functions. As CD44 has been reported to show different activated states between monocytes and macrophages, we propose that CD44 may be involved in monocyte differentiation. In this study, we explored the role of CD44 in monocyte differentiation and further studied the mechanisms that were involved in. THP-1 cells (human monocytic leukemia cell line) were induced with phorbol 12-myristate 13-acetate (PMA) to establish the model of monocyte differentiation in vitro. It was found that CD44 expression and binding capacity to hyaluronic acid were increased significantly, and the distribution of CD44 was converted into clusters during differentiation. The PMA-induced CD44 clustering and CD44 high expression were suppressed by blocking CD44, which resulted in the inhibition of CD14 expression. PMA-induced phosphorylation of ERK1/2 signal was also suppressed by blocking CD44. Our results suggested that CD44 was involved in monocyte differentiation. The mechanisms of monocyte differentiation following CD44 activation may include CD44 high expression and clustering which in turn lead to phosphorylation of ERK1/2.