Transcriptomic responses to darkness stress point to common coral bleaching mechanisms
Transcriptomic responses to darkness stress point to common coral bleaching mechanisms
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DOI：
10.1007/s00338-011-0833-4
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发表时间：
2012-03-01
期刊：
影响因子：
3.5
通讯作者：
Medina, Monica
中科院分区：
文献类型：
--
作者：
DeSalvo, M. K.;Estrada, A.;Medina, Monica
珊瑚白化是对许多非生物压力的反应，其中与生态最相关的是由于海水温度升高而引起的高温压力。漂白事件可以跨越很大的地理区域，目前是对全世界珊瑚礁的一个突出威胁。许多努力都集中在了解漂白的分子和细胞事件，这些研究主要利用热和光应力制度。为了确定不同的应激源是否具有共同的漂白机制，我们使用了珊瑚Acropora palmata和Montastraea faveolata的互补DNA（cDNA）微阵列（包含> 10，000个特征）来测量黑暗应激期间的差异基因表达。我们的研究结果揭示了一个惊人的转录组反应黑暗的A。掌状体涉及伴侣蛋白和抗氧化剂上调，生长停滞和代谢修饰。由于这些反应是以前在热应力测量，我们的研究结果表明，不同的压力可能有共同的漂白机制。此外，我们的研究结果指出，缺氧和内质网应激作为关键的细胞事件参与分子漂白机制。另一方面，我们在M. faveolata中，宿主菌落和取样位置之间的基因表达差异大于对照和应激片段之间的差异。这和以前的珊瑚微阵列研究揭示了巨大的范围内的转录组学反应，是可能的，当研究两种珊瑚物种，在其生态生理学差异很大，从而指出预测珊瑚将如何应对未来的环境变化的比较方法的重要性。
Coral bleaching occurs in response to numerous abiotic stressors, the ecologically most relevant of which is hyperthermic stress due to increasing seawater temperatures. Bleaching events can span large geographic areas and are currently a salient threat to coral reefs worldwide. Much effort has been focused on understanding the molecular and cellular events underlying bleaching, and these studies have mainly utilized heat and light stress regimes. In an effort to determine whether different stressors share common bleaching mechanisms, we used complementary DNA (cDNA) microarrays for the corals Acropora palmata and Montastraea faveolata (containing >10,000 features) to measure differential gene expression during darkness stress. Our results reveal a striking transcriptomic response to darkness in A. palmata involving chaperone and antioxidant up-regulation, growth arrest, and metabolic modifications. As these responses were previously measured during thermal stress, our results suggest that different stressors may share common bleaching mechanisms. Furthermore, our results point to hypoxia and endoplasmic reticulum stress as critical cellular events involved in molecular bleaching mechanisms. On the other hand, we identified a meager transcriptomic response to darkness in M. faveolata where gene expression differences between host colonies and sampling locations were greater than differences between control and stressed fragments. This and previous coral microarray studies reveal the immense range of transcriptomic responses that are possible when studying two coral species that differ greatly in their ecophysiology, thus pointing to the importance of comparative approaches in forecasting how corals will respond to future environmental change.