P53 GENE IS UP-REGULATED DURING SKELETAL-MUSCLE CELL-DIFFERENTIATION
P53 GENE IS UP-REGULATED DURING SKELETAL-MUSCLE CELL-DIFFERENTIATION
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DOI：
10.1006/bbrc.1993.1473
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发表时间：
1993-04-30
影响因子：
3.1
通讯作者：
HALEVY, O
中科院分区：
文献类型：
--
作者：
HALEVY, O
小鼠肌原细胞系 C2 或鸡卫星细胞原代培养物中的细胞分化是由生长培养基中的低血清水平诱导的。孵育约5小时后，内源野生型p53 mRNA大量表达。孵育 18 小时后，在用 10−8M 视黄酸处理的细胞中也观察到 p53 mRNA 表达的诱导。在这两种情况下，p53 mRNA 的增加都是短暂的。 c-fos mRNA 水平迅速下降，在接触视黄酸 2 小时后几乎检测不到。 c-fos 的下调证实了其在肌肉细胞分化中的作用，而野生型 p53 的上调则表明了其在此过程中的作用。
Differentiation of cells in the mouse myogenic cell line C2, or a primary culture of chicken satellite cells was induced by low serum levels in the growth medium. Endogenous wild-type p53 mRNA was substantially expressed after approximately 5 h of incubation. Induction of p53 mRNA expression was also observed in cells treated with 10−8M retinoic acid, after 18 h of incubation. In both cases, the increase in p53 mRNA was transient. c-fos mRNA levels decreased rapidly and were barely detectable after 2 h of exposure to retinoic acid. The down-regulation of c-fos confirms its role in muscle cell differentiation, whereas the up-regulation of wild-type p53 suggests its role during this process.