Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB
Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB
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DOI：
10.1128/aem.03006-05
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发表时间：
2006-07-01
影响因子：
4.4
通讯作者：
Andersen, G. L.
中科院分区：
文献类型：
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作者：
DeSantis, T. Z.;Hugenholtz, P.;Andersen, G. L.
16S rRNA基因数据库（http://greengenes.bl.gov）通过提供嵌合体筛选、标准比对和使用多个已发布的分类方法进行分类，解决了公共存储库的限制。研究发现，即使在门水平上，策展人之间也存在着不一致的分类命名。在3%的环境序列和0.2%的分离株记录中发现了假定的嵌合体。环境序列在古细菌和细菌中被划分为100个门级谱系。
A 16S rRNA gene database (http://greengenes.bl.gov) addresses limitations of public repositories by providing chimera screening, standard alignment, and taxonomic classification using multiple published taxonomies. It was found that there is incongruent taxonomic nomenclature among curators even at the phylum level. Putative chimeras were identified in 3% of environmental sequences and in 0.2% of records derived from isolates. Environmental sequences were classified into 100 phylum-level lineages in the Archaea and Bacteria.