Polyurethane membrane/knitted mesh-reinforced collagen–chitosan bilayer dermal substitute for the repair of full-thickness skin defects via a two-step procedure
Polyurethane membrane/knitted mesh-reinforced collagen–chitosan bilayer dermal substitute for the repair of full-thickness skin defects via a two-step procedure
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聚氨酯膜/针织网状增强胶原蛋白-壳聚糖双层真皮替代品，通过两步程序修复全层皮肤缺损
DOI：
10.1016/j.jmbbm.2015.11.021
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发表时间：
2016
影响因子：
3.9
通讯作者：
Chunmao Han
中科院分区：
文献类型：
--
作者：
Xingang Wang;Pan Wu;Xiuyuan Hu;Chuangang You;Rui Guo;Haifei Shi;Songxue Guo;Hanlei Zhou;Yu Chaoheng;Yuanhai Zhang;Chunmao Han
真皮替代物的出现为深层皮肤缺损的修复和重建提供了革命性的策略。真皮替代物形成再生模板，其提供引导细胞迁移、细胞外基质（ECM）沉积和血管生成所必需的多孔结构和机械支撑。商业上可获得的真皮替代物，特别是基于胶原蛋白的真皮支架，广泛用于临床实践。然而，基于胶原蛋白的真皮支架的较差的机械性能损害了其生物学效应以及修复结果。在这里，我们描述了一种双层真皮替代品，通过将混合真皮支架与聚氨酯（PU）膜整合，以获得PU膜/针织网增强胶原蛋白-壳聚糖双层真皮替代品（PU-PLGAm/CCS）。研究了PU-PLGAm/CCS的形态学，并且为了表征PU-PLGAm/CCS对组织再生的影响，使用两步手术程序移植真皮替代物以修复Sprague-Dawley大鼠中的全层皮肤伤口。然后将这些结果与使用PELNAC™人工真皮获得的结果进行比较。在第一次手术后的几周内，根据肉眼观察分析伤口变化，并收获组织标本进行组织学、免疫组织化学、免疫荧光实时定量PCR和Western印迹分析。在第二操作（即，移植断层厚度皮肤移植物），基于机械强度和ECM表达研究修复结果。PU-PLGAm/CCS显著抑制伤口挛缩，促进血管生成，并促进新组织的有序排列，使得PU-PLGAm/CCS组的修复结果与PELNAC™组相比得到改善。总之，真皮替代物良好的微观结构和结构稳定性有利于组织再生。PU-PLGAm/CCS结合了生物材料和合成材料的优点，实现了多孔结构、生物相容性和力学性能之间的平衡，具有良好的皮肤组织工程应用前景。
The advent of dermal substitutes provides a revolutionary strategy for the repair and reconstruction of deep skin defects. Dermal substitutes form a regenerative template that provides the porous structure and mechanical support necessary to guide cell migration, deposition of the extracellular matrix (ECM) and angiogenesis. Commercially available dermal substitutes, particularly collagen-based dermal scaffolds, are widely used in clinical practice. However, the poor mechanical properties of collagen-based dermal scaffolds compromise their biological effects, as well as the repair outcomes. Here, we describe a bilayer dermal substitute prepared by integrating a hybrid dermal scaffold with a polyurethane (PU) membrane to obtain a PU membrane/knitted mesh-reinforced collagen–chitosan bilayer dermal substitute (PU-PLGAm/CCS). The morphology of PU-PLGAm/CCS was investigated and, to characterize the effects of PU-PLGAm/CCS on tissue regeneration, dermal substitutes were transplanted to repair full-thickness skin wounds in Sprague-Dawley rats using a two-step surgical procedure. These results were then compared with those obtained using the PELNAC™ Artificial Dermis. In the weeks after the first operation, wound changes were analysed based on macroscopic observations, and tissue specimens were harvested for histology, immunohistochemistry, immunofluorescence real-time quantitative PCR, and Western blotting analysis. Following the second operation (i.e., transplantation of split-thickness skin grafts), the repair outcomes were investigated based on the mechanical strength and ECM expression. PU-PLGAm/CCS significantly inhibited wound contracture, promoted angiogenesis, and facilitated the ordered arrangement of neotissue, such that the repair outcomes were improved in the PU-PLGAm/CCS group compared with the PELNAC™ group. In conclusion, the favourable microstructure and structural stability of dermal substitutes facilitated tissue regeneration. PU-PLGAm/CCS achieved a balance between porous structure, biocompatibility and mechanical properties for dermal regeneration by integrating the advantages of biological and synthetic biomaterials, which demonstrates its potential for skin tissue engineering.