AFM detects the effects of acidic condition on the size and biomechanical properties of native/oxidized low-density lipoprotein.
AFM detects the effects of acidic condition on the size and biomechanical properties of native/oxidized low-density lipoprotein.
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DOI：
10.1016/j.colsurfb.2021.112053
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发表时间：
2021-08
期刊：
影响因子：
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通讯作者：
Kun Wang;Chaoye Gan;Huaying Wang;Mei Ao;Youlong Fan;Yong Chen
中科院分区：
文献类型：
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作者：
Kun Wang;Chaoye Gan;Huaying Wang;Mei Ao;Youlong Fan;Yong Chen
溶液酸化存在于某些生理条件下（如细胞中的溶酶体）和疾病（如动脉粥样硬化、肿瘤等）。酸化是否以及如何影响天然低密度脂蛋白（LDL）及其氧化形式（oxLDL）的大小和生物力学（硬度和粘性）特性，这在动脉粥样硬化和肿瘤发生中起着至关重要的作用，目前还知之甚少。原子力显微镜（AFM）评估，从pH 7.4到pH 4.4的梯度酸化引起的膨胀，第一和然后收缩的大小和刚度的急剧下降（但没有统计学上显着的变化粘性）的LDL/oxLDL颗粒通过影响二级/三级结构和脂质释放检测的红外光谱分析和胆固醇检测，分别。LDL/oxLDL在酸性条件下相对于在中性pH下更小和更软的特性部分解释了酸化的致动脉粥样硬化作用。这些数据可能为更好地了解LDL/oxLDL和某些疾病（如动脉粥样硬化和肿瘤）提供重要信息。
Solution acidification exists under some physiological conditions (e.g. lysosomes in cells) and diseases (e.g. atherosclerosis, tumors, etc.). It is poorly understood whether and how acidification influences the size and biomechanical (stiffness and stickiness) properties of native Low-density lipoprotein (LDL) and its oxidized form (oxLDL) which plays a vital role in atherogenesis and tumorigenesis. Atomic force microscopy (AFM) evaluated that gradient acidification from pH 7.4 to pH 4.4 caused an expanding-first-and-then-shrinking decrease in size and a dramatic decrease in stiffness (but no statistically significant changes in stickiness) of LDL/oxLDL particles by influencing secondary/tertiary structures and lipid release detected by infrared spectral analysis and cholesterol detection, respectively. The smaller and softer characteristics of LDL/oxLDL at acidic conditions versus at the neutral pH partially explains the atherogenic role of acidification. The data may provide important information for a better understanding of LDL/oxLDL and some diseases (e.g. atherosclerosis and tumors).