Immunoassay for pathogenic bacteria using platinum nanoparticles and a hand-held hydrogen detector as transducer. Application to the detection of Escherichia coli O157:H7
Immunoassay for pathogenic bacteria using platinum nanoparticles and a hand-held hydrogen detector as transducer. Application to the detection of Escherichia coli O157:H7
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使用铂纳米颗粒和手持式氢检测器作为传感器对病原菌进行免疫测定。在大肠杆菌O157:H7检测中的应用
DOI：
10.1007/s00604-019-3409-6
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发表时间：
2019-05-01
影响因子：
5.7
通讯作者：
Wan, Jia-Yu
中科院分区：
文献类型：
--
作者：
Bu, Sheng-Jun;Wang, Kui-Yu;Wan, Jia-Yu
提出了一种用于便携式和灵敏的病原菌检测的创新方法。一种新型的合成杂化纳米复合材料包裹铂纳米颗粒，作为一种高效的催化剂，催化氨-硼烷复合物水解产生氢气。纳米复合材料被用作免疫测定的标记。便携式手持式氢检测器结合纳米复合材料诱导的信号转换应用于病原菌的即时检测。手持式氢检测器被用作传感器。大肠杆菌O 157：H7（E. coli O 157：H7）作为检测靶，与磁珠和杂化纳米复合材料形成三明治结构。采用磁珠分离夹心结构，并将杂化纳米复合材料作为催化剂催化氨硼烷制氢。使用电化学方法通过氢检测器检测所产生的氢。E.大肠杆菌O 157：H7的检出限为10 CFU mL（-1）。该免疫传感器使手持式氢检测器成为户外使用的即时检测仪，用于检测和定量氢以外的目标。
An innovative approach is presented for portable and sensitive detection of pathogenic bacteria. A novel synthetic hybrid nanocomposite encapsulating platinum nanoparticles, as a highly efficient catalyst, catalyzes thehydrolysis of the ammonia-borane complex to generate hydrogen gas. The nanocomposites are used as a label for immunoassays. A portable hand-held hydrogen detector combined with nanocomposite-induced signal conversion was applied for point-of-care testingofpathogenic bacteria. A hand-held hydrogen detector was used as the transducer. Escherichia coli O157:H7 (E. coli O157: H7), as detection target, formed a sandwich structure with magnetic beads and hybrid nanocomposites. Magnetic beads were used for separation of the sandwich structure, and hybrid nanocomposites as catalysts to catalyze the generation of hydrogen from ammonia-borane. The generated hydrogen was detected by a hydrogen detector using an electrochemical method. E. coli O157:H7 has a detection limit of 10CFUmL(-1). The immunosensor made the hand-held hydrogen detector a point-of-care meter to be used outdoors for the detection and quantification of targets beyond hydrogen.