Insight on a comprehensive profile of volatile compounds of Chlorella vulgaris extracted by two “green” methods
Insight on a comprehensive profile of volatile compounds of Chlorella vulgaris extracted by two “green” methods
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两种“绿色”方法提取的小球藻挥发性化合物的综合概况
作者：
C. Lafarge;N. Cayot
摘要对普通小球藻不同样品中挥发性化合物的提取方法进行了筛选和试验：环境友好型超声辅助液液萃取(LLE)和固相微萃取(SPME)。得到的挥发性化合物的图谱不同。两种萃取物中只有一个分子是相同的。利用固相微萃取方法，鉴定出的挥发性化合物的主要化学类别是硫化物、醛和醇。使用LLE方法，挥发物的分布与烷烃、脂肪酸、萜类、醇和醛更平衡。多变量数据分析允许在样本之间进行区分。此外，还研究了挥发性化合物的物化性质与提取方法的关系。结果表明，液液萃取可以提取高沸点(>；160℃)和高logP(>；3)的挥发性化合物。固相微萃取法对低沸点(<；160℃)和低logP(<；3)的挥发性化合物的提取更为有效。因此，有必要将几种提取方法结合起来，以获得微藻样品挥发性分布的完整视图。
Abstract Some green extraction methods were selected and tested for the extraction of volatile compounds from different samples of the microalga Chlorella vulgaris: ultrasound‐assisted liquid–liquid extraction using environment‐friendly solvents (LLE) and solid‐phase microextraction (SPME). The obtained profiles of volatile chemical compounds were different. Only one molecule was found in common to both extractions. Using the SPME method, the main chemical classes of identified volatile compounds were sulfuric compounds, aldehydes, and alcohols. Using the LLE method, the volatile profile was more balanced with alkanes, fatty acids, terpenes, alcohols, and aldehydes. Multivariate data analyses permitted discrimination among samples. Additionally, the relationship between the physicochemical properties of identified volatile compounds and the methods of extraction was studied. The results showed that the LLE extraction allowed the extraction of volatile compounds having a high boiling point (>160°C) and a high log P (>3). The SPME method was more effective to extract volatile compounds with a low boiling point (<160°C) and a low log P (<3). It is thus necessary to combine several extraction methods to obtain a complete view of the volatile profile for microalgae samples.