RELIABILITY OF PHENOTYPIC TESTS FOR IDENTIFICATION OF ACINETOBACTER SPECIES
RELIABILITY OF PHENOTYPIC TESTS FOR IDENTIFICATION OF ACINETOBACTER SPECIES
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DOI：
10.1128/jcm.29.2.277-282.1991
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发表时间：
1991-02-01
影响因子：
9.4
通讯作者：
URSING, J
中科院分区：
文献类型：
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作者：
GERNERSMIDT, P;TJERNBERG, I;URSING, J
使用数值方法来鉴定根据 Tjernberg 和 Ursing 的分类分配到 DNA 组的 198 个不动杆菌菌株（I. Tjernberg 和 J. Ursing，APMIS 97：595-605，1989）。 使用的矩阵是根据 Bouvet 和 Grimont (P.J.M. Bouvet and P.A.D. Grimont, Int. J. Syst. Bacteriol. 36:228-240, 1986) 和 Bouvet 和 Jeanjean (P.J.M. Bouvet and S. Jeanjean, Res. Microbiol. 140:291-299, Res. Microbiol. 140:291-299, 1989）。 所选择的测试是 Bouvet 和 Grimont 设计的不动杆菌属简化鉴定方案（P.J.M. Bouvet 和 P.A.D. Grimont, Ann. Inst. Pasteur/Microbiol. 138:569-578, 1987），即在 37、41 和 44 摄氏度下生长、葡萄糖氧化、明胶水解和同化14 种碳源。 在测试的菌株中，181 个代表基质中的 12 个 DNA 组；在大于或等于0.95的概率水平下，其中78%被正确识别，2.2%被错误识别，19.8%未被识别。 17 个菌株代表两个未包含在矩阵中的 DNA 组；通过这些表型测试，其中九个被错误地分配到 DNA 组。 由于使用 Bouvet 和 Grimont (Ann. Inst. Pasteur/Microbiol.) 提出的表型测试分离属于 DNA 组 1、2、3 和 13 的菌株存在问题，我们建议这些组应称为 Acinetobacter calcoacetus-A。鲍曼氏菌复合体。
A numerical approach was used for identification of 198 Acinetobacter strains assigned to DNA groups according to the classification of Tjernberg and Ursing (I. Tjernberg and J. Ursing, APMIS 97:595-605, 1989). The matrix used was constructed from data published by Bouvet and Grimont (P.J.M. Bouvet and P.A.D. Grimont, Int. J. Syst. Bacteriol. 36:228-240, 1986) and Bouvet and Jeanjean (P.J.M. Bouvet and S. Jeanjean, Res. Microbiol. 140:291-299, 1989). The test chosen were those of the simplified identification scheme for Acinetobacter species devised by Bouvet and Grimont (P.J.M. Bouvet and P.A.D. Grimont, Ann. Inst. Pasteur/Microbiol. 138:569-578, 1987), namely, growth at 37, 41, and 44-degrees-C, oxidation of glucose, gelatin hydrolysis, and assimilation of 14 carbon sources. Of the strains tested, 181 represented 12 DNA groups in the matrix; at a probability level of greater-than-or-equal-to 0.95, 78% of them were correctly identified, 2.2% were misidentified, and 19.8% were not identified. Seventeen strains represented two DNA groups not included in the matrix; nine of them were incorrectly assigned to a DNA group by these phenotypic tests. Because of problems of separating strains belonging to DNA groups 1,2,3, and 13 by using the phenotypic tests proposed by Bouvet and Grimont (Ann. Inst. Pasteur/Microbiol.), we suggest that these groups should be referred to as the Acinetobacter calcoaceticus-A. baumannii complex.