Feasibility of using lignin isolated from forages by solubilization in acetyl bromide as a standard for lignin analyses.
Feasibility of using lignin isolated from forages by solubilization in acetyl bromide as a standard for lignin analyses.
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DOI：
10.2527/2000.78123135x
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发表时间：
2000-12
影响因子：
3.3
通讯作者：
R. S. Fukushima;B. A. Dehority
中科院分区：
文献类型：
--
作者：
R. S. Fukushima;B. A. Dehority
木质素浓度可以通过乙酰溴可溶性木质素分光光度法测量;然而，与任何分光光度法一样，需要可靠的标准。在本实验中，用乙酰溴试剂从研究中的每种牧草中提取木质素。然后将用乙酰溴（LIAB）分离的木质素用作乙酰溴可溶性木质素（ABSL）分析中的参考标准，将其与酸洗涤剂木质素（ADL）和高锰酸钾木质素（PerL）木质素分析进行比较。分析了以下每种牧草的两个成熟期：紫花苜蓿、狗牙根、紫花苜蓿、狗牙根和紫花苜蓿。Coastal，Panicum maximum var. Centenário和var. Colonião、Cynodon plectostachyus、Pennisetum purpureum、狗尾草南迪和燕麦。此外，一个木材样品，桉树，进行了分析总体上ABSL值最高（P < 0.001），其次是PerL和ADL，两者之间也有显著性差异（P < 0.001）。与干物质体外消化率的相关性以ABSL法最高。LIAB的吸收光谱，无论是从植物的不同成熟阶段或从不同的蔬菜品种，建议存在的差异之间的一些木质素。
Lignin concentration can be measured in plants by the acetyl bromide-soluble lignin spectrophotometric method; however, as with any spectrophotometric method, a reliable standard is needed. In the present experiments, lignin was extracted from each of the forages under study with the acetyl bromide reagent. The lignin isolated with acetyl bromide (LIAB) was then used as the reference standard in the acetyl bromide-soluble lignin (ABSL) analysis, which was compared with the acid detergent lignin (ADL) and potassium permanganate lignin (PerL) lignin analyses. Two maturity stages of each of the following forages were analyzed: Medicago sativa, Cynodon dactylon var. Coastal, Panicum maximum var. Centenário and var. Colonião, Cynodon plectostachyus, Pennisetum purpureum, Setaria nandi, and Avena sativa. In addition, one wood sample, Eucalyptus sp., was analyzed. In general, ABSL values were highest (P < 0.001), followed by PerL and ADL, which also differed from each other (P < 0.001). Correlations with in vitro dry matter digestibility of samples were highest with the ABSL method. Absorption spectra of LIAB, either from plants of different maturity stages or from different vegetable species, suggested the presence of differences among some of the lignins.