Transcriptional Mechanism of Jasmonate Receptor COI1-Mediated Delay of Flowering Time in Arabidopsis
Transcriptional Mechanism of Jasmonate Receptor COI1-Mediated Delay of Flowering Time in Arabidopsis
复制标题
茉莉酸受体COI1介导拟南芥开花时间延迟的转录机制
DOI：
10.1105/tpc.15.00619
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发表时间：
2015
期刊：
影响因子：
11.6
通讯作者：
Li Chuanyou
中科院分区：
文献类型：
--
作者：
Zhai Qingzhe;Zhang Xin;Wu Fangming;Feng Hailong;Deng Lei;Xu Li;Zhang Min;Wang Qiaomei;Li Chuanyou
植物的开花时间必须严格控制，以最大限度地繁殖成功。植物已经进化出复杂的信号网络来协调开花的时间，以响应不断变化的环境条件。除了作为一个关键的免疫信号，脂质衍生的植物激素茉莉酸（JA）还调节广泛的发育过程，包括开花时间。在此，我们报道了COI 1依赖的信号通路通过抑制成花基因FLOWERLOCUS T（FT）的表达来延迟拟南芥的开花时间。我们提供了遗传和生物化学证据表明，APETALA 2转录因子（TF）的目标EAT 1（TOE 1）和TOE 2与JAZ（茉莉酸-ZIM结构域）蛋白的子集相互作用，并抑制FT的转录。我们的研究结果支持这样一种情况，即当植物遇到胁迫条件下，生物活性JA促进COI 1依赖的JAZ降解。JAZ阻遏物的降解释放了TOE的转录功能，从而抑制FT的表达，从而触发信号级联反应，延迟开花。我们的研究确定了相互作用的TF和JAZ转录调节因子对，这些转录调节因子是JA介导的开花调节的基础，这表明JA信号通过匹配TF和JAZ蛋白质对转化为特定的上下文依赖性反应。
Flowering time of plants must be tightly regulated to maximize reproductive success. Plants have evolved sophisticated signaling network to coordinate the timing of flowering in response to their ever-changing environmental conditions. Besides being a key immune signal, the lipid-derived plant hormone jasmonate (JA) also regulates a wide range of developmental processes including flowering time. Here, we report that the CORONATINE INSENSITIVE1 (COI1)-dependent signaling pathway delays the flowering time ofArabidopsis thalianaby inhibiting the expression of the florigen geneFLOWERING LOCUS T(FT). We provide genetic and biochemical evidence that the APETALA2 transcription factors (TFs) TARGET OF EAT1 (TOE1) and TOE2 interact with a subset of JAZ (jasmonate-ZIM domain) proteins and repress the transcription ofFT. Our results support a scenario that, when plants encounter stress conditions, bioactive JA promotes COI1-dependent degradation of JAZs. Degradation of the JAZ repressors liberates the transcriptional function of the TOEs to repress the expression ofFTand thereby triggers the signaling cascades to delay flowering. Our study identified interacting pairs of TF and JAZ transcriptional regulators that underlie JA-mediated regulation of flowering, suggesting that JA signals are converted into specific context-dependent responses by matching pairs of TF and JAZ proteins.