2 DIFFERENT FORMS OF BETA MYOSIN HEAVY-CHAIN ARE EXPRESSED IN HUMAN STRIATED-MUSCLE
2 DIFFERENT FORMS OF BETA MYOSIN HEAVY-CHAIN ARE EXPRESSED IN HUMAN STRIATED-MUSCLE
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DOI：
10.1007/bf00272378
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发表时间：
1987-10-01
期刊：
影响因子：
5.3
通讯作者：
LIEW, CC
中科院分区：
文献类型：
--
作者：
JANDRESKI, MA;SOLE, MJ;LIEW, CC
我们在人类身上发现了两条β样肌球蛋白重链的证据，一条是心脏的，一条是骨骼的。将心脏β肌球蛋白重链cDNA克隆pHMC3和骨骼β样肌球蛋白重链cDNA克隆pSMHCZ的cDNA序列相互比较。发现3''非翻译区以及指定羧基编码区的482个核苷酸是100%同源的。进一步检查表明，骨骼克隆 pSMHCZ 在 5'' 端与人心脏 β 肌球蛋白重链 cDNA 克隆 pHMC3 不同。我们在本报告中提供的证据表明，心脏 β 肌球蛋白重链 mRNA 在骨骼肌组织中表达。人心脏 β 肌球蛋白重链 cDNA 克隆 pHMC3 编码肌球蛋白重链轻片段肌球蛋白部分的一部分，被用作 S1 核酸酶图谱研究的探针，其 RNA 来源于心脏组织、平滑肌和骨骼肌组织，包括快肌纤维、慢肌纤维以及混合的快肌纤维和慢肌纤维。使用两个探针来检查 mRNA 的表达。一个探针（406 个核苷酸）构成 β 心肌肌球蛋白重链 cDNA 克隆的 3'' 非翻译区和部分编码区，与骨骼 cDNA 克隆 pSMHCZ 100% 同源。另一个构成心脏克隆pHMC3的大部分编码区（1017个核苷酸），其中标记末端的前216个核苷酸与骨骼克隆pSMHCZ 100%同源。在富含慢肌I型肌纤维的比目鱼肌中，心脏β肌球蛋白重链mRNA的表达非常显着。在腓肠肌（一种混合纤维肌肉）中，检测到的该 mRNA 的表达程度低于比目鱼肌。在主要由 II 型快肌纤维组成的股外侧肌和股内侧肌中，存在微量的心脏 β 肌球蛋白重链 mRNA。当在平滑肌组织中测试该 mRNA 的表达时，没有检测到任何结果。
We have found evidence for two beta-like myosin heavy chains in humans, one cardiac and one skeletal. The cDNA sequence of the cardiac beta myosin heavy chain cDNA clone pHMC3 and the skeletal beta-like myosin heavy chain cDNA clone pSMHCZ, were compared to each other. It was found that the 3'' untranslated regions as well as 482 nucleotides specifying the carboxyl coding region, were 100% homologous. Further examination revealed that the skeletal clone pSMHCZ diverges from the human cardiac beta myosin heavy chain cDNA clone pHMC3 at the 5'' end. We present evidence in this report which indicates that the cardiac beta myosin heavy chain mRNA is expressed in skeletal muscle tissues. The human cardiac beta myosin heavy chain cDNA clone, pHMC3, which codes for a portion of the light meromyosin section of the myosin heavy chain, was used as a probe for S1 nuclease mapping studies with RNA derived from cardiac tissue, smooth muscle and skeletal muscle tissues consisting of fast-twitch, slow-twitch and mixed fast- and slow-twitch muscle fibres. Two probes were used to examine the expression of the mRNA. One probe (406 nucleotides) constitutes the 3'' untranslated region and a portion of the coding region of the beta cardiac myosin heavy chain cDNA clone, which is 100% homologous to pSMHCZ, the skeletal cDNA clone. The other constitutes the majority of the coding region (1017 nucleotides) of the cardiac clone pHMC3 in which the first 216 nucleotides from the labelled end are 100% homologous to the skeletal clone pSMHCZ. In the soleus muscle, which is rich in slow-twitch type I muscle fibres, the expression of the cardiac beta myosin heavy chain mRNA was very prominent. In gastrocnemius muscle, a mixed fibre muscle, the expression of this mRNA was detected to a lesser degree than that for the soleus muscle. In vastus lateralis and vastus medialis, which consist of predominantly type II, fast-twitch fibres, there were trace amounts of the cardiac beta myosin heavy chain mRNA. When expression of this mRNA was tested in smooth muscle tissue none could be detected.