Stability of RNA sequences derived from the coronavirus genome in human cells
Stability of RNA sequences derived from the coronavirus genome in human cells
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DOI：
10.1016/j.bbrc.2020.05.008
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发表时间：
2020-07-05
影响因子：
3.1
通讯作者：
Akimitsu, Nobuyoshi
中科院分区：
文献类型：
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作者：
Wakida, Hiroyasu;Kawata, Kentaro;Akimitsu, Nobuyoshi
大多数病毒抑制宿主细胞的先天免疫系统和/或RNA降解过程，以构建有利于其存活的细胞内环境。RNA病毒基因组内的特征RNA序列或从DNA病毒基因组转录的RNA有助于这种抑制。在这项研究中，我们开发了一种名为“Fate-seq”的方法，以全面识别来自RNA和DNA病毒的RNA序列，从而有助于细胞中的RNA稳定性。我们使用融合在GFP报告RNA下游的这些RNA的下一代测序，检查了来自26种不同病毒（16种RNA病毒和10种DNA病毒）的5，924个RNA片段的稳定活性。通过Fate-seq方法，我们检测到了多种稳定GFP报告RNA的病毒衍生RNA序列，包括源自严重急性呼吸道综合征相关冠状病毒（SARS-CoV）的序列。比较基因组分析显示，这些RNA序列及其预测的二级结构在SARS-CoV和新型冠状病毒SARS-CoV-2之间高度保守，后者是2019年12月出现的冠状病毒相关疾病全球爆发的原因（COVID-19）。这些序列具有增强病毒RNA基因组稳定性的潜力，从而增强病毒复制效率和毒力。(C)2020爱思唯尔公司All rights reserved.
Most viruses inhibit the innate immune system and/or the RNA degradation processes of host cells to construct an advantageous intracellular environment for their survival. Characteristic RNA sequences within RNA virus genomes or RNAs transcribed from DNA virus genomes contribute toward this inhibition. In this study, we developed a method called "Fate-seq" to comprehensively identify the RNA sequences derived from RNA and DNA viruses, contributing RNA stability in the cells. We examined the stabilization activity of 5,924 RNA fragments derived from 26 different viruses (16 RNA viruses and 10 DNA viruses) using next-generation sequencing of these RNAs fused 30 downstream of GFP reporter RNA. With the Fate-seq approach, we detected multiple virus-derived RNA sequences that stabilized GFP reporter RNA, including sequences derived from severe acute respiratory syndrome-related coronavirus (SARS-CoV). Comparative genomic analysis revealed that these RNA sequences and their predicted secondary structures are highly conserved between SARS-CoV and the novel coronavirus, SARS-CoV-2, which is responsible for the global outbreak of the coronavirus-associated disease that emerged in December 2019 (COVID-19). These sequences have the potential to enhance the stability of viral RNA genomes, thereby augmenting viral replication efficiency and virulence. (C) 2020 Elsevier Inc. All rights reserved.