Clock mutant mice with Jcl/ICR background shows an impaired learning ability in water maze, but not in passive avoidance, at the beginning of dark phase
Clock mutant mice with Jcl/ICR background shows an impaired learning ability in water maze, but not in passive avoidance, at the beginning of dark phase
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DOI：
10.1111/j.1741-4520.2006.00107.x
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发表时间：
2006-06
影响因子：
1.3
通讯作者：
H. Sei;K. Oishi;A. Sano;H. Seno;T. Ohmori;Y. Morita;N. Ishida
中科院分区：
文献类型：
--
作者：
H. Sei;K. Oishi;A. Sano;H. Seno;T. Ohmori;Y. Morita;N. Ishida
摘要我们观察了具有JCL/ICR背景的时钟突变小鼠(ClockJ)在暗期早期的学习能力。用Morris水迷宫(WM)和被动回避(PA)试验评价大鼠的学习能力。用体内微透析法测定海马乙酰胆碱、5-羟色氨酸(5-HT)和多巴胺(DA)的释放量。ClockJ在西医测验中表现为学习能力减退，而在PA测验中未见。与野生型小鼠相比，ClockJ组小鼠海马乙酰胆碱释放显著减少。海马区的5-羟色胺和多巴胺都不受时钟突变的影响。Clock是一个控制昼夜节律的重要基因，可能对空间学习和海马胆碱能功能有重要作用，至少在暗期开始时是这样。
ABSTRACT We observed the learning ability in Clock mutant mice with Jcl/ICR background (Clockj), a mice model of evening‐type individuals, in the early part of dark phase. In order to estimate the learning ability, Morris water maze (WM) and passive avoidance (PA) test were performed. Release of acetylcholine, 5 hydroxytryptophan (5‐HT) and dopamine (DA) in hippocampus was measured by in vivo microdialysis method. Clockj showed the impaired learning ability in the WM, but not in PA test. Hippocampal acetylcholine release was significantly attenuated in the Clockj in comparison to the wild‐type mice. Neither 5‐HT nor DA in the hippocampus was affected by the Clock mutation. Clock, an essential gene controlling circadian rhythm, may have an important role on the spatial learning and hippocampal cholinergic function, at least, at the beginning of the dark phase.