Differential pulmonic NK and NKT cell responses in Schistosoma japonicum-infected mice
Differential pulmonic NK and NKT cell responses in Schistosoma japonicum-infected mice
复制标题
DOI：
10.1007/s00436-016-5320-y
复制
发表时间：
2017-02-01
影响因子：
2
通讯作者：
Huang, Jun
中科院分区：
文献类型：
--
作者：
Cha, Hefei;Qin, Wenjuan;Huang, Jun
自然杀伤细胞（NK细胞）和自然杀伤T细胞（NKT细胞）在抗感染、抗肿瘤、移植免疫和自身免疫调节中发挥作用。然而，NK和NKT细胞在日本血吸虫（S. japonicum）感染中的作用尚未被广泛报道，特别是在肺部感染方面。本研究的目的是研究NK细胞和NKT细胞对小鼠肺部日本血吸虫感染的反应。免疫荧光组织学分析显示，肺肉芽肿附近可见NK和NKT细胞。流式细胞术检测结果显示，日本血吸虫感染小鼠肺NK细胞百分比和数量均显著升高（P < 0.05）。但与正常小鼠相比，NKT细胞百分比和细胞数明显减少（P < 0.05）。感染后肺NK细胞和NKT细胞上CD69的表达升高（P < 0.05），而CD25仅在NKT细胞上表达升高（P < 0.05）。细胞内细胞因子染色显示，与对照组相比，ifn - γ(+)百分比较高，IL-5（+）百分比较低（P < 0.05）。而IL-17（+）、IL-10（+）、IL-5（+）肺NKT细胞比例显著升高（P < 0.05）。肺NKT细胞NKG2A/C/E （CD94）表达显著降低（P < 0.05）， NKG2D （CD314）表达显著升高（P < 0.05），这可能是日本血吸虫感染时NKT细胞活化的机制之一。
Natural killer cells (NK cells) and natural killer T cells (NKT cells) play a role in anti-infection, anti-tumor, transplantation immunity, and autoimmune regulation. However, the role of NK and NKT cells during Schistosoma japonicum (S. japonicum) infection has not been widely reported, especially regarding lung infections. The aim of this study was to research the NK and NKT cell response to S. japonicum infection in the lungs of mice. Using immunofluorescent histological analysis, NK and NKT cells were found near pulmonary granulomas. Moreover, flow cytometry revealed that the percentage and number of pulmonic NK cells in S. japonicum-infected mice were significantly increased (P < 0.05). However, the percentage and cell number of NKT cells were decreased compared to those of normal mice (P < 0.05). The expression of CD69 on pulmonic NK and NKT cells was increased after infection (P < 0.05), and CD25 expression increased only on NKT cells (P < 0.05). Intracellular cytokine staining showed a higher percentage of IFN-gamma(+) and lower percentage of IL-5(+) pulmonic NK cells (P < 0.05) compared to controls. However, the percentage of IL-17(+), IL-10(+), and IL-5(+) pulmonic NKT cells significantly increased (P < 0.05). Additionally, there was a significant decrease in NKG2A/C/E (CD94) expression and an increase of NKG2D (CD314) expression on pulmonic NKT cells (P < 0.05), which might serve as a mechanism for NKT cell activation during S. japonicum infection.