Immunohistochemical nuclear staining for p53, PCNA, and Ki-67 in different histologic variants of basal cell carcinoma
Immunohistochemical nuclear staining for p53, PCNA, and Ki-67 in different histologic variants of basal cell carcinoma
复制标题
DOI：
10.1016/s0190-9622(97)70145-2
复制
发表时间：
1997-09-01
影响因子：
13.8
通讯作者：
Skelton, HG
中科院分区：
文献类型：
--
作者：
Barrett, TL;Smith, KJ;Skelton, HG
背景：在大多数基底细胞癌(BCC)中已发现P53的表达增加；然而，只有大约50%的病例存在紫外线诱导的标志性突变。目的：探讨不同组织学类型的膀胱移行细胞癌与其P53、增殖细胞核抗原和Ki-67的表达是否有关系。方法：采用免疫组织化学方法，对浅表多中心型、结节溃变型、硬化型、浸润型和不典型型BCC进行P53、增殖细胞核抗原和Ki-67的免疫组织化学染色，以确定在不同的组织学类型的BCC中是否存在不同的染色模式。结节状基底细胞癌呈中等强度的P53核染色，并伴有一定程度的周边强化。结节状基底细胞癌中，增殖细胞核抗原染色大于Ki-67，阳性细胞数小于10%。硬化型和浸润型基底细胞癌P53核染色较强，周边强化，增殖细胞核抗原染色强于Ki-67，大部分阳性细胞大于30%。不典型基底细胞癌P53呈弥漫性强染色。在所研究的所有肿瘤中，增殖细胞核抗原染色均大于Ki-67，且阳性细胞数均大于30%，当上复光化性角化病显示P53染色时，其染色与癌旁组织中阳性染色的强弱甚至阳性与否并不一定相关。结论：P53、PCNA和Ki-67的染色至少有四种不同的模式与不同的组织学类型有关。
Background: Increased expression of p53 has been found in the majority of basal cell carcinomas (BCCs); however, UV-light-induced signature mutations are present in only about 50% of eases. Increased nuclear staining with an immunohistochemical marker of proliferation, proliferating cell nuclear antigen (PCNA), has been correlated with aggressive behavior in BCC.Objective: Our purpose was to determine whether there is any relationship between different histologic variants of BCC and, their expression of p53, PCNA, and Ki-67.Methods: We used immunohistochemical stains for p53, PCNA, and Ki-67, in superficial-multicentric, nodular-noduloulcerative, sclerosing, infiltrative, and metatypical BCC, to determine whether the staining patterns differ in these different histologic variants of BCC.Results: Superficial-multicentric BCCs were negative for p53 in four of eight tumors. Nodular BCC showed moderately intense p53 nuclear staining with some peripheral accentuation. PCNA nuclear staining was greater than Ki-67, and PCNA-positive cells were fewer than 10% in nodular BCC. Sclerosing and infiltrative BCC showed intense p53 nuclear staining with peripheral accentuation, PCNA nuclear staining was greater than Ki-67, and PCNA-positive cells were greater than 30% in the majority of these tumors. Metatypical BCCs showed diffuse intense p53 staining. PCNA nuclear staining was greater than Ki-67, and PCNA-positive cells were greater than 30% in all tumors studied, When overlying actinic keratoses showed p53 staining, the staining did not necessarily correlate with the intensity or even the presence of positive staining in the subjacent BCC.Conclusion: There are at least four distinctive patterns for staining of p53, PCNA, and Ki-67 that correlate with different histologic variants of BCC.