The Semen pH Affects Sperm Motility and Capacitation.
The Semen pH Affects Sperm Motility and Capacitation.
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DOI：
10.1371/journal.pone.0132974
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yao B
中科院分区：
文献类型：
--
作者：
Zhou J;Chen L;Li J;Li H;Hong Z;Xie M;Chen S;Yao B
由于精液的化学环境会对精子质量产生深远的影响，我们研究了 pH 值对人类精子的活力、活力和获能的影响。本研究中的精子是从健康男性身上采集的，以避免其他因素的干扰。对在pH 5.2、6.2、7.2和8.2的精子营养液中培养的精子进行精子总活力、前向活力(PR)、低渗膨胀(HOS)率和精子穿透力的分析。我们的结果表明，这些参数在 pH 7.2 和 8.2 精子营养液中相似，但在 pH 5.2 和 6.2 溶液中下降。 HOS率与精子总活力和PR呈正相关。此外，还测量了不同pH值下精子Na+/K+-ATP酶活性，结果发现，与pH 8.2和pH 7.2溶液相比，在pH 5.2和6.2介质中酶活性显着降低。使用流式细胞术（FCM）和激光共聚焦扫描显微镜（LCSM）分析，测定在pH 5.2、6.2、7.2和8.2的精子获能溶液中培养的精子的细胞内Ca2+浓度。与pH 7.2时相比，pH 5.2和6.2培养基中精子的平均荧光强度显着降低，而pH 8.2组则无差异。我们的研究结果表明，酸性 pH 值下 Na+/K+-ATP 酶活性下降会导致精子运动和获能减少，这可能是男性不育的机制之一。
As the chemical environment of semen can have a profound effect on sperm quality, we examined the effect of pH on the motility, viability and capacitation of human sperm. The sperm in this study was collected from healthy males to avoid interference from other factors. The spermatozoa cultured in sperm nutrition solution at pH 5.2, 6.2, 7.2 and 8.2 were analyzed for sperm total motility, progressive motility (PR), hypo-osmotic swelling (HOS) rate, and sperm penetration. Our results showed that these parameters were similar in pH 7.2 and 8.2 sperm nutrition solutions, but decreased in pH 5.2 and 6.2 solutions. The HOS rate exhibited positive correlation with the sperm total motility and PR. In addition, the sperm Na+/K+-ATPase activity at different pHs was measured, and the enzyme activity was significantly lower in pH 5.2 and 6.2 media, comparing with that in pH 8.2 and pH 7.2 solutions. Using flow cytometry (FCM) and laser confocal scanning microscopy (LCSM) analysis, the intracellular Ca2+ concentrations of sperm cultured in sperm capacitation solution at pH 5.2, 6.2, 7.2 and 8.2 were determined. Compared with that at pH 7.2, the mean fluorescence intensity of sperm in pH 5.2 and 6.2 media decreased significantly, while that of pH 8.2 group showed no difference. Our results suggested that the declined Na+/K+-ATPase activity at acidic pHs result in decreased sperm movement and capacitation, which could be one of the mechanisms of male infertility.