Evidence for circadian rhythms in human trophoblast cell line that persist in hypoxia
Evidence for circadian rhythms in human trophoblast cell line that persist in hypoxia
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DOI：
10.1016/j.bbrc.2008.11.006
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发表时间：
2009-01-02
影响因子：
3.1
通讯作者：
Bertolucci, Cristiano
中科院分区：
文献类型：
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作者：
Frigato, Elena;Lunghi, Laura;Bertolucci, Cristiano
昼夜节律钟控制着许多生理过程如生殖功能的每日节律性。胎盘中是否存在生物钟尚不清楚。为了研究人类胎盘是否可以作为昼夜节律振荡器，我们利用HTR-8/SVneo细胞来源于人类早期妊娠滋养层。在血清休克细胞中，我们发现时钟基因Per 2和Dec 1以及经典时钟控制基因DBP的昼夜节律表达。我们获得了类似的结果为VEGF，昼夜输出参与控制胎盘血管发生和滋养层功能。有趣的是，昼夜节律振荡持续存在，甚至在实验性CoCl 2缺氧的细胞中增强。这些结果可以解释，因为早期妊娠胎盘的缺氧环境被认为是正常胎盘形成的最佳条件。这些数据共同支持VEGF的差异节律表达的可能作用，受昼夜节律钟的影响，在胎盘血管化和滋养层功能的调整，以适应不同胎龄的具体要求。(C)2008年爱思唯尔公司All rights reserved.
Circadian clock governs daily rhythmicity of a number of physiological processes such as reproductive functions. The existence of circadian clocks in the placenta is not clearly established. In order to investigate whether human placenta may function as circadian oscillator, we utilized HTR-8/SVneo cells derived from human first-trimester trophoblast. In serum-shocked cells we found circadian expressions for the clock genes Per2 and Dec1 as well as for Dbp, a canonical clock-controlled gene. We obtained similar results for Vegf, a circadian output involved in the control of placental vasculogenesis and trophoblast functions. Interestingly, circadian oscillations persisted and even enhanced in cells experimentally rendered hypoxic with CoCl2. These results could be explained since the hypoxic milieu of the first-trimester placenta is considered the optimal condition for normal placentation. These data collectively Support a possible role for the differential rhythmic expression of Vegf, influenced by circadian clock, in the adjustment of placental vascularization and trophoblast functions to the specific requirements of the different gestational ages. (C) 2008 Elsevier Inc. All rights reserved.