Detection of sub-nanogram quantities of Mojave toxin via enzyme immunoassay with light addressable potentiometric detector.
Detection of sub-nanogram quantities of Mojave toxin via enzyme immunoassay with light addressable potentiometric detector.
复制标题
使用光可寻址电位检测器通过酶免疫分析检测亚纳克量的莫哈韦毒素。
DOI：
10.1016/0956-5663(93)80060-3
复制
发表时间：
1993
影响因子：
12.6
通讯作者：
Chambers,JP
中科院分区：
文献类型：
--
作者：
Colston,JT;Kumar,P;Rael,ED;Tsin,AT;Valdes,JJ;Chambers,JP
Sensitive detection of Mojave toxin (MoTX), a potent neurotoxin isolated from the venom ofCrotalus scutulatus scutulatus, was developed using an enzyme immunoassay (EIA) and light addressable potentiometric detection. This EIA utilizes both biotin-and fluorescein-labeled anti-MoTX antibodies to immobilize and detect sub-nanogram to nanogram quantities of toxin. Labeled mono- and polyclonal anti-MoTX antibodies were used alone and/or in combination to determine maximum assay sensitivity. Assays performed using a combination of biotinylated poly-and fluoresceinated monoclonal antibodies produced an assay with a lower detection limit near 2.5 ng. Assays performed using labeled polyclonals alone, or in combination with boitinylated mono-and fluoresceinated polyclonal antibodies, indicated increased sensitivity with a detection limit near 300 pg. In conclusion, we describe an enzyme immuno-assay using different labeled antibody schemes which detects sub-nanogram quantities of MoTX via light addressable potentiometric detection.
