Detection of ricin using a carbon nanofiber based biosensor
Detection of ricin using a carbon nanofiber based biosensor
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DOI：
10.1016/j.bios.2011.07.061
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发表时间：
2011-10-15
影响因子：
12.6
通讯作者：
Koehne, Jessica E.
中科院分区：
文献类型：
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作者：
Periyakaruppan, Adaikkappan;Arumugam, Prabhu U.;Koehne, Jessica E.
我们报告蓖麻毒素检测使用抗体和适配体探针固定在纳米电极阵列（NEA）组成的垂直排列的碳纳米纤维（VACNF）。这些生物传感器芯片使用集成电路制造中常见的步骤在晶片级上制造。电化学阻抗谱被用来表征检测事件，结果表明，电子传递电阻的变化后，蓖麻蛋白结合到探针显着。通过原子力显微镜对电极表面的评价获得了进一步的确认，原子力显微镜清楚地显示了从裸电极到由探针-蛋白质结合的表面的高度变化。由爱思唯尔公司出版
We report ricin detection using antibody and aptamer probes immobilized on a nanoelectrode array (NEA) consisting of vertically aligned carbon nanofibers (VACNFs). These biosensor chips are fabricated on a wafer scale using steps common in integrated circuit manufacturing. Electrochemical impedance spectroscopy is used to characterize the detection event and the results indicate that the electron transfer resistance changes significantly after the ricin protein binds to the probe. Further confirmation is obtained from evaluation of the electrode surface by atomic force microscopy which clearly shows a change in height from the bare electrode to the surface bound by the probe-protein. Published by Elsevier B.V.