A continuous spectrophotometric assay for pyrimidine-5'-nucleotidase.
A continuous spectrophotometric assay for pyrimidine-5'-nucleotidase.
复制标题
嘧啶-5-核苷酸酶的连续分光光度测定。
DOI：
10.1016/0003-2697(85)90176-9
复制
发表时间：
1985
影响因子：
2.9
通讯作者：
Tanaka,KR
中科院分区：
文献类型：
--
作者：
Zerez,CR;Tanaka,KR
提出了一种用连续分光光度分析系统测定嘧啶-5‘-核苷酸酶活性的简便方法。活性是通过使用在肌苷、嘌呤核苷磷酸化酶和黄嘌呤氧化酶存在的情况下产生尿酸的链接指示系统来测量无机磷生成来确定的。与目前使用的任何方法相比，这种方法有几个优点。
A simple method is presented for the determination of pyrimidine-5′-nucleotidase activity using a continuous spectrophotometric assay system. Activity is determined by measuring inorganic phosphate generation using a linked indicator system that produces uric acid in the presence of inosine, purine nucleoside phosphorylase, and xanthine oxidase. This method has several advantages over any of the methods currently in use.