The differential expression of galectin-1 and galectin-3 in normal lymphoid tissue and non-Hodgkin's and Hodgkin's lymphomas
The differential expression of galectin-1 and galectin-3 in normal lymphoid tissue and non-Hodgkin's and Hodgkin's lymphomas
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DOI：
10.1177/039463200501800304
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发表时间：
2005-07-01
影响因子：
3.5
通讯作者：
Salmon, I
中科院分区：
文献类型：
--
作者：
D'Haene, N;Maris, C;Salmon, I
淋巴瘤的WHO分类建立在临床、形态学、免疫组化和遗传标准的基础上。然而，每个实体都显示出自己的临床攻击性谱。治疗的成功率差异很大，无法预测。由于半乳糖凝集素参与肿瘤发生和免疫细胞的生理学，我们研究了半乳糖凝集素-1和galectin-3的免疫组织化学表达是否可以在25个正常淋巴组织，42个非霍奇金淋巴瘤和14个霍奇金淋巴瘤不同。半乳糖凝集素表达的免疫组织化学提交半定量和定量（计算机辅助显微镜）评价。本研究通过分析（通过定量RT-PCR）3个正常淋巴结、3个滤泡性淋巴瘤（FL）和3个弥漫性大B细胞淋巴瘤（DLBCL）中的半乳糖凝集素-3mRNA表达来完成。数据显示，在正常淋巴组织中，淋巴细胞不表达半乳糖凝集素-1，并且很少表达半乳糖凝集素-3。与此相反，galectin-3在16例DLBCL中的8例和8例FL中的1例中表达。此外，半乳糖凝集素-3 mRNA在DLBCL中的表达是FL中的3倍。虽然淋巴瘤的血管壁表达半乳糖凝集素-1，但正常淋巴组织的血管壁不表达。半乳糖凝集素-1在血管壁中的表达与血管密度相关。因此，本研究表明，可以根据半乳凝素-3的表达将DLBCL与正常淋巴组织和其他淋巴瘤区分开来。
The WHO classification of lymphomas was established on the basis of clinical, morphological, immunohistochemical and genetic criteria. However, each entity displays its own spectrum of clinical aggressiveness. Treatment success varies widely and is not predictable. Since galectins are involved in oncogenesis and the physiology of immune cells, we investigated whether galectin-1 and galectin-3 immunohistochemical expression could differ in 25 normal lymphoid tissues, 42 non-Hodgkin's and 14 Hodgkin's lymphomas. Immunohistochemical galectin expression was submitted to semi-quantitative and quantitative (computer-assisted microscopy) evaluations. This study is completed by an analysis (by means of quantitative RT-PCR) of galectin-3 mRNA expression in 3 normal lymph nodes, 3 follicular lymphomas (FLs) and 3 diffuse large B-cell lymphomas (DLBCLs). The data show that in normal lymphoid tissue, lymphocytes do not express galectin-1 and rarely express galectin-3. In contrast, galectin-3 was expressed in 8 of the 16 DLBCL cases and in I of the 8 FL cases. Furthermore, galectin-3 mRNA was expressed 3-times more in the DLBCLs than in the FLs. While the blood vessel walls of the lymphomas expressed galectin-1, the vessel walls of normal lymphoid tissues did not. This expression of galectin-1 in blood vessel walls was correlated with vascular density. The present study thus shows that DLBCL can be distinguished from normal lymphoid tissue and other lymphomas on the basis of galectin-3 expression.