Volumetric motility-contrast imaging of tissue response to cytoskeletal anti-cancer drugs
Volumetric motility-contrast imaging of tissue response to cytoskeletal anti-cancer drugs
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DOI：
10.1364/oe.15.014057
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发表时间：
2007-10-17
期刊：
影响因子：
3.8
通讯作者：
Nolte, David D.
中科院分区：
文献类型：
--
作者：
Jeong, Kwan;Turek, John J.;Nolte, David D.
细胞运动的显微成像最近已经从二维发展到三维，用于药物开发。然而，随着成像深度的增加，分辨率显著降低，限制了从样品内部深处获取运动信息。在此，数字全息光学相干成像适于允许在常规运动性测定方法无法达到的深度处的组织中的运动性的可视化。该方法使用肿瘤组织内的运动性的时程测量来跟踪细胞骨架抗癌药物对其天然三维环境内的组织的影响。(c)2007年，美国光学学会。
Microscopic imaging of cellular motility has recently advanced from two dimensions to three dimensions for applications in drug development. However, significant degradation in resolution occurs with increasing imaging depth, limiting access to motility information from deep inside the sample. Here, digital holographic optical coherence imaging is adapted to allow visualization of motility in tissue at depths inaccessible to conventional motility assay approaches. This method tracks the effect of cytoskeletal anti-cancer drugs on tissue inside its natural three-dimensional environment using time-course measurement of motility within tumor tissue. (c) 2007 Optical Society of America.